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Purpose: Many Korean college students suffer from physical inactivity and mental health problems. However, it has not been sufficiently reported how this lack of exercise and health-related behavior affect their health. The present study was performed to identify the relationships among physical activity level, health-promoting behavior, and physiological variables in Korean undergraduate and graduate students. Methods: Participants were 115 undergraduate and graduate students from one university in Seoul. The Pearson's correlation analysis was performed using SPSS for Windows. Results: Physical activity level had significant positive correlations with health-promoting behavior (r=.32, p=.001) and exercise self-efficacy (r=.25, p=.008), and health-promoting behavior had a significant correlation with depression (r=-.33, p<.001) and exercise self-efficacy (r=.44, p<.001). Additionally, physical activity level had significant correlations with triglyceride (r=-.20, p=.034) and vitamin D (r=.20, p=.029) levels. The high density cholesterol level had significant negative correlations with systolic blood pressure (r=-.33, p<.001), diastolic blood pressure (r=-.29, p=.002), and vitamin D (r=-.20, p=.035) levels. Conclusion: Physical activity level or health-promoting behavior had significant relationships with the health status of college students. Strategies need to be developed to improve health-promoting behaviors among college students.