The main factor in the pathogenesis oftraamatic and burn damage to eye tissues is the disruption of regeneration processes, which may lead to detrimental consequences such as rough cicatrization [1]. Clinical practice (in particular, ophthalmologic) constantly suffers from a lack of medicinals capable of accelerating the reparative processes in tissues. A promising direction in the development of drugs possessing these properties consists in the use of natural biostimulators, Glycosaminoglycans (GAGs) are the components of connective tissues that take an active part both in the tissue metabolism and in the stimulation of reparative processes during traumatic and bum damage [2]. Histochemical investigations showed a sharp drop in the corneal GAG content upon damage to the eye. As is known, the main GAG fractions entering into the composition of eye cornea are chondroitin sulfate (CS) and keratan sulfate (KS) necessary for recovery of the transparency of eye cornea) [3 -6].The drug keracol, developed in the Ufa Research Institute of Eye Diseases and produced by the "Immunopreparat" corporation (Ufa Department), provides clarification of the cornea following various kinds of damage including bums, ulcers, fistulas, decementocytosis, and mechanical injury. However, the keracol substance, obtained from the bovine cornea by a process involving special treatment and comminution, exhibits a number of disadvantages. The drug is insoluble in water, rapidly washed out as a result of lacrimation, and can be used for therapy only under stationary hospital conditions. All this stimulated development of a new drug, free of the above disadvantages and exceeding keracol in its therapeutic efficacy. Below we present the results of investigation of the process of GAG extraction from bovine cornea and the pharmacological properties of eye drops prepared on the basis of the new drug.

Chondroitin sulfates are important natural polysaccharides widely occurring in animal tissues in the form of salts or neutral complexes with collagen and other proteins [1]. The interest in this class of carbohydrates is currently increasing because preparations based on chondroitin sulfates have been successfully used for the therapy of some diseases. For example, Russian drug chonsurid is used for the treatment of slowl-healing operative wounds, trophic ulcers, and extremivy varicosis [2]. Chondroitin sulfate based preparations are also used in the therapy of rheumatic diseases [2], in the manufacture of contact lenses [3] and artificial blood vessels [4], and for the production of bandages of the "synthetic leather" type [5]. Finally, chondroitin sulfates are the main components in some medicinal cosmetic preparations [6].Quite readily available and comparatively cheap raw materials obtained from animals for the production of chondroitin sulfates are factors stimulating the activity of research. At present, raw materials of animal orig-in (mostly from bovine trachea) are still the main sources of chondroitin sulfates. In order to meet the needs in chondroitin sulfate preparations, it is necessary to extend the circle of raw material sources so as to produce ehondroitin sulfates in appropriate amounts and intensify research aimed at the creation of new preparations with combined action.One possible source of chondroitin sulfates is the trachea of reindeer (Rangifer tarandus), which are currently not utilized. Unfortunately, neither chemical structure not the phar-macolo~cal activity of ehondroitin sulfates obtained from reindeer trachea were reported in the literature. The purpose of this work was to fill this gap. EXPERIMENTAL CHEMICAL PARTThe IR spectra were measured on a UR-20 spectrophotometer using samples dissolved in D20. The specific op-77 tical rotation was studied with a Perkin-Eimer Model 141 polarimeter. The UV spectra of 0.5 % aqueous solutions of chondroitin sulfates obtained from reindeer and bovine materials were recorded on a Speeord M-40 spectrophotometer. The chondroitin sulfates were isolated from the trachea of both types using a conventional method described in [7]. The contents ofhexoses, ~ucuronie acid, and proteins were determined as described in [8 -10]. The expedrnents were performed using a vertical electrophoresis apparatus using 0.9% agarose gel (Sigma) in 0.I M BaAc2 (pH 8.0). The IPS-600 power source was operated at a current of 80 mA and a voltage of 55 V. The reference samples was represented by chondroitin sulfates of the AC and B grade (Sigma Chemical Company) and heparin (Moscow Endocrine Plant). The gel was stained by a 1% solution of toluidine blue in 1% aqueous acetic acid.

Data on the synthesis and properties of a new affine sorbent created on the basis of a modified poly(acrylamide) gel with immobilized m-aminophenylboronic acid are presented. The new sorbent can be used for a multiple (no less then 7 cycles) quantitative determination of compounds containing cis-diol groups, in particular, glycosylated hemoglobin. The proposed affine sorbent offers a promising basis for the creation of diagnostic screening systems of carbohydrate metabolism disturbances and for monitoring diabetes mellitus compensation. In a series of tests for glycosylated hemoglobin determination with the new sorbent, the variation coefficient did not exceed 5%.