No abstract

Camel is an important source of food as well as of transport for large communities in sub-Saharan Africa, the Middle East, and the Indian subcontinent. The present study aimed to evaluate the effect of different concentrations of honey (0.0, 1.0, 2.5, or 5.0%) addition to Tris-extender on epididymal camel semen quality during storage at 5º C for up to 48 hrs. Eleven healthy dromedary camels (Camelus dromedarius), aged between 8 to 15 years, were used in the present study during the rutting season. Percentages of sperm motility, dead spermatozoa, abnormal spermatozoa, acrosome damage, and membrane integrity were determined. The obtained results showed that the extended semen supplemented with honey at levels of 1%, and 2.5% significantly (P<0.01) improved percentages of sperm motility, intact acrosome, and membrane integrity, while decreased the percentages of dead and abnormal camel spermatozoa during storage at 5ºC for up to 48 hrs. Moreover, the addition of 5.0% honey to the extender significantly (P<0.01) tended to be a deleterious effect on camel semen quality. In conclusion, Tris-extender added with 2.5% honey improved camel semen quality during storage at 5º C for up to 48 hrs.

Eighteen ejaculates were collected from three mature Arabian stallions (about 5 years of age) during breeding season. Semen was extended with Tris-yolk fructose extender immediately after collection and evaluated. Diluted semen (0.1 ml) was added to 0.9 ml of Na-citrate-lactose solution to get a final concentration of (50, 100, 150, 200 and 300 mOsm/Kg-1) and then incubated at 37˚C for up to 60 minutes. After each incubation time (0, 5, 15, 30 and 60 minutes), the response of the stallion spermatozoa to HOS-test was assessed and the percentage of sperm motility, percentage of spermatozoa with intact acrosome, percentage of spermatozoa with coiled tails and swollen spermatozoa were estimated. The obtained results revealed that, the percentages of motile spermatozoa and spermatozoa with intact acrosomes were significantly (P<0.05) increased, while the percentages of spermatozoa with coiled tails and swollen spermatozoa were significantly (P<0.05) decreased in the extended spermatozoa with Na-citrate-lactose solution at a level of 300 mOsm/Kg-1 as compared to 50, 100, 150 and 200 mOsm/ Kg-1 , during the incubation at 37ºC for up to 60 minutes. The prolongation of the time of incubation at 37ºC for up to 60 minutes of the extended spermatozoa decreased significantly (P<0.05) the percentages of motile spermatozoa and spermatozoa with intact acrosomes, while increased significantly (P<0.05) the percentages of spermatozoa with coiled tails and swollen spermatozoa extended with Na-citrate-lactose solution at a level of 300 mOsm/ Kg-1 compared to 50, 100, 150 and 200 mOsm/ Kg-1. In conclusion, HOSt could be recommended for semen evaluation in stallions because it is applicable and inexpensive.

No abstract

Stem cell therapy is considered an important and innovative tool for applied research in andrology, especially in infertility; therefore, it was later adapted as potential therapeutic agents. This study aimed to evaluate rat mononuclear bone marrow cells' ability to recover testis cells in cyclophosphamide (CTX)-treated rats and to assess its effects on hormonal and histopathological changes. The bone marrow cells were harvested from femurs and tibias of rats and purified by a Histopaque gradient. Mononuclear bone marrow cells transplantation was performed by intravenous injection of cells in cyclophosphamide-treated animals. Three weeks after transplantation, blood samples were collected and analyzed for hormonal assay. In addition, the testes were collected for histological and histopathological determination. The results depicted that the serum levels of all tested hormones were significantly different among the three experimental groups G1 (control, healthy animals), G2 (CTX induced infertility and untreated) and G3 (CTX induced infertility and treated with stem cells). FSH and LH levels were significantly increased in G2 (CTX) compared to G1 and G3. Total and free testosterone levels were slightly higher in G3 compared to G2. Mononuclear bone marrow cell transplantation promoted cellular reorganization of the seminiferous epithelium. Also, spermatogenesis regeneration was improved. In conclusion, bone marrow stem cells can regenerate the damaged testicular elements and hence restore hormonal regulation in cyclophosphamide treated rat. Therefore, the treatment of male infertility and testosterone deficiency could be therapeutically treated by using stem cells.