Isolation and identification of arbuscular mycorrhizal fungi (AMF) in cashew plants was carried out in Datah Village, Abang District of Karangasem Regency. This study aims to determine the diversity of mycorrhizal spores in the cashew rhizosphere and AMF infections in the roots of cashew plants. The result in microscopic observations found 5 genera of AMF spores such as Glomus, Racocetra, Entrophospora, Septoglomus, and Acaulospora and the population of the genus Glomus were found most dominant in the rhizosphere of cashew plants. The result of staining root with trypan blue obtained the internal structure of AMF in the form of vesicles, arbuscules and hyphae found in root tissue of cashew plants. The result of AMF DNA amplification with primer NS31 and AML2 showed 560 bp bands in electrophoresis with 1% agarose gel determined AMF infection in root of cashew plants.
Artificial diet for insect rearing is necessary at the time a lot of insects required periodically and continuously such as for pesticide efficacy research, biodiversity conservation, and for the activity of fruit fly management with the sterile male technique for instance where it needs millions fruit flies weekly to be sterilized and then subsequently released. Insect artificial diet will also increase the efficiency of time, energy and fund in the procurement of insects compared to insect rearing with its natural diet. Various artificial diet classifications and its compositions have been developed so far in varying degrees of rearing success.
Marigold flower has many benefits in many aspects of life in Bali Island (Indonesia) including often used in religious ceremonial activities. For controlling plant pests and diseases, marigold is planted on the sidelines or on the edge of a land as an attractant crop to invite natural enemies and marigold also can produce certain substance which can reduce the incidence of nematode. In inducing mutagenesis in this research, the seeds of marigold were soaked in solution of colchicine with hydrogen peroxide as its solvent. The results of this research indicated that the treatments caused the formation of chlorophyll mutant and an increase in the length of flower diameter. Both the control and treated plant samples were attacked by leaf miner, giant land snail, virus-like disease and botrytis flower blight disease.
Agas merupakan kelompok lalat kecil yang mempunyai kemampuan terbang yang termasuk dalam beberapa famili, banyak genus dan banyak sekali spesies. Agas sciarid yang dibahas dalam artikel ulasan ini di Bali disebut lulut yang mempunyai nilai spiritual dimana apabila ada kelompok larva agas keluar dari dalam tanah ke permukaan tanah maka ritual khusus akan dilakukan oleh pemilik lahan pada lokasi tersebut. Banyak pihak yang bertanya-tanya, lulut itu binatang jenis apa?. Artikel ulasan ini mencoba menjawab pertanyaan tersebut diatas. Berbagai hal dibahas dari aspek entomologi sampai aspek pertanian, diantaranya: morfologi, siklus hidup, habitat, agregasi, migrasi, posisinya sebagai hama tanaman dan juga cara pengendaliannya.
Colchicine treatments of 0.005% and 0.01% either in distilled water or in hydrogen peroxide 3% were caused temporary leaf shape changes on cowpea. Treatment of colchicine in hydrogen peroxide 3% resulted in green pod color change to purple however treatment of just hydrogen peroxide 3% and colchicine in hydrogen peroxide 3% caused 8.8-10.7% dwarf cowpea. As a preliminary data, we also observed that cowpea crops grown from seeds treated with colchicine in distillate water attacked by bean leaf beetle much earlier compare to the treatment with colchicine in hydrogen peroxide 3%.
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