The KATRIN experiment aims at a direct and model independent determination of the neutrino mass with 0.2 eV/c 2 sensitivity (at 90% C.L.) via a measurement of the endpoint region of the tritium beta-decay spectrum. The main components of the experiment are a windowless gaseous tritium source (WGTS), differential and cryogenic pumping sections and a tandem of a preand a main-spectrometer, applying the concept of magnetic adiabatic collimation with an electrostatic retardation potential to analyze the energy of beta decay electrons and to guide electrons passing the filter onto a segmented silicon PIN detector. One of the important systematic uncertainties of such an experiment are due to energy losses of β-decay electrons by elastic and inelastic scattering off tritium molecules within the source volume which alter the shape of the measured spectrum. To correct for these effects an independent measurement of the corresponding energy loss function is required. In this work we describe a deconvolution method to extract the energy loss function from measurements of the response function of the experiment at different column densities of the WGTS using a monoenergetic electron source.