A method of Agrobacterium-mediated genetic transformation of sugarbeet (Beta vulgaris L.) with vacuum infiltration has been developed. Aseptic 3-weeks old etiolated seedlings of two diploid O-type sugarbeet lines (KS3 and KS7) have been used for genetic transformation. Transgenic sugarbeet plants carrying the reporter beta-glucuronidase gene have been selected for their resistance to glufosinate ammonium herbicide. Integration of transgenes into sugarbeet genome was confirmed with GUS assay and PCR using primers for bar and gusA genes.

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Phosphinothricin-resistant plants of commercial summer rapeseed varieties ( Brassica napus L. var. oleifera DC) were obtained by means of Agrobacterium tumefaciens-mediated transformation of leaf disks. The vector structures contained the promoterless coding sequence of the phosphinothricin acetyltransferase gene ( bar ), which is situated between two inverted lox sites (elements of the Cre/ lox recombination systems of the PI phage) and the selective neomycinphosphotransferase II gene ( nptII ). The presence of the newly introduced genes in the genome of the obtained plants is confirmed by the polymerase chain reaction method. Stable and linked inheritance of these genes in the T 1 and T 2 generations is demonstrated.