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In an experimentally established model of torsion of the testis, morphological and immunological observations were evaluated in 180 Wistar rats. Torsion of the testis were carried out to observe in animals the biological phenomenon that naturally occurs in man. The effect of the twisted testis on the contralateral testis was carefully checked. The morphological observations revealed serious damage to the seminiferous tubules in the contralateral testis. In four rats, persistent infertility was observed as an effect of torsion. The infertility was confirmed by total atrophy of seminiferous epithelium in testis section. The cell-binding ability of sera obtained from rats at different times after torsion were studied by cell-binding radioimmunoassay with different types of germinal cells. The presence of autoantibodies to epididymal spermatozoa was revealed in most cases, being a secondary effect of the thus-far unknown, nonspecific factors responsible for damage of the contralateral testis.

Using a modified hot plate method for measuring analgesia in rats, the analgesic dose of morphine was estimated to be about 5 mg./kg. This dose increased by 50 per cent the time the animals withstood exposure before reacting to the thermal stimulation. When chlor‐promazine 1.5 mg./kg. was given simultaneously with morphine, a similar degree of analgesia was obtained with about 2 mg./kg. of the narcotic. The sensitivity decreased markedly in rats receiving daily injections of analgesic doses of morphine. Chlorpromazine did not retard the development of tolerance to morphine in these rats. Some of the toxic effects of morphine, however, were less marked in the rats treated with both drugs than those treated with morphine alone.

Experimental torsions of the testicle were carried out in 100 adult and 80 premature Wistar rats. Histology revealed in 27.7% severe morphological changes in the contralateral testicles. Their possible influence on temporal or irreversible disturbances of fertility is discussed. CB-RIA investigations detected the presence of antisperm antibodies in the serum of 67 between 90 rats of the experimental group.