To study the seroprevalence of Lawsonia intracellularis among the pig livestock of Ukraine. The research was conducted in the laboratories of the Institute of Veterinary Medicine of NAAS and in pig farms. Were studied 653 samples of pigs’ serum of different age groups from17 pig farms with breeding stock of 40 456 sows. To study seroprevalence was used a test system for enzyme-linked immunosorbent assay based on monoclonal antibodies bioScreen Ileitis Antibody Test ELISA Svanovir®Lawsonia intracellularis (Boehringer Ingelheim Svanova). Serological tests have provided evidence that the pathogen was circulating in the herd. According to the research results, 46.4% of seropositive animals were found. Seropositive pigs were found in all studied farms, namely – among sows – 74.0%, replacement gilts – 79.6%, growing pigs – 2.5% and fattening pigs – 59.8%. The high level of seroprevalence among replacement gilts and sows of the 1st farrowing indicates the circulation of the pathogen in the herd, as well as the manifestation of morbidity in the fattening group, where was the highest rate of positive serum samples. From the 17 examined farms, only one farm was found where no seropositive animals were detected. According to our observations, proliferative enteropathy in pigs of Ukrainian pig farms has signs of chronic course, especially it is common in pig farms that import replacement gilts from abroad, where antibiotics are prohibited or restricted in donor farms. The results of serological tests allowed us to reconfirm that the spread of the disease is increasing in Ukrainian pig farms. Compared to 2015, the percentage of seropositive animals increased by almost 10%. The typical serological profile for Lawsonia intracellularis which is characteristic of our studied farms allowed us to conclude for which age groups of pigs and for which technological period of raising swine it is necessary to develop and implement measures for treatment and disease prevention. To control the disease, it is highly recommended to conduct constant monitoring of the farm’s well-being against ileitis (send, in addition to faecal samples for PCR testing, also serum for ELISA testing).

Інститут ветеринарної медицини ВИГОТОВЛЕННЯ ПЕРОКСИДАЗНОГО КОН'ЮГАТУ ДЛЯ ІФА ДІАГНОСТИКИ ЛЕЙКОЗУ ВЕЛИКОЇ РОГАТОЇ ХУДОБИ У статті викладено результати виготовлення пероксидазного кон'югату рекомбінантного білка G Streptococcus spp. для діагностичної лейкозної тест-системи на основі твердофазного варіанту імуноферментного аналізу. Для отримання кон'югату був застосований перйодатний метод. Встановлено оптимальне розведення імуноферментного кон'югату, а саме 1:32000 -1:64000, яке визначали шляхом кратних послідовних розведень під час постановки ІФА зі заздалегідь відомими позитивною та негативною на лейкоз великої рогатої худоби сироватками крові. Ключові слова: пероксидазний кон'югат, білок G Streptococcus spp., імуноферментний аналіз, лейкоз великої рогатої худоби,.

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