Maximum oxygen consumption (VO2 max) and flexibility are essential biomotoric components for football athletes. The influence of genetics on the biomotoric element has not been widely studied. The ACTN3 gene probably affects VO2 max and flexibility. This study aims to determine the relationship between ACTN3 gene polymorphism to VO2 max and flexibility in students of UKM Olahraga Jenderal Soedirman University. This was an observational analytic study with a cross-sectional approach. Seventy-eight subjects chosen by consecutive sampling underwent the protocol study. Venous blood samples were taken for ACTN3 gene polymorphism examination. The respondents were also tested for VO2 max with the multi-stage fitness test and flexibility tests using sit and reach method. Data were analyzed by a one way ANOVA test with a significance level of p=0.05.The results of this study showed that there was no significant relationship between ACTN3 gene polymorphism with VO2 max (p=0.763) and flexibility (p=0.912). The highest mean VO2 max was in the RR genotype (35.25±7.15 ml/kg.min), while the highest mean of flexibility was in the XX genotype (37.02±7.89 cm). There was no relationship between ACTN3 gene polymorphism with VO2 max and flexibility, in students of Jenderal Soedirman University.

Results: Twenty patients were accrued. 55% was male. All patients had clinical T3 N positive disease except one had T4 disease. Pathological response was evaluable in 19 patients. Dworak TRG 1-2 and 3-4 were detected in 36.8% and 63.2% respectively. After median follow-up of 5.93 years, five patients (25%) developed recurrent distant metastatic disease and finally expired. No recurrent disease occurred after 2.39 years. The 5-year DFS and OS were 75% and 80% respectively. Although no statistically significant difference was found, patients with Dworak TRG 1-2 had lower 5-year DFS and OS than those with TRG 3-4 (TRG 1-2, DFS 66.7% vs. TRG 3-4, DFS 100%, p¼.058 and TRG 1-2, OS 66.7% vs. TRG 3-4, OS 100%, p¼.059 respectively). At present, all patients with Dworak TRG 3-4 are alive and have never had recurrent disease. Conclusions: Most locally advanced rectal cancer patients receiving preoperative concurrent CapeOx/RT achieved best Dworak TRG. Long-term outcomes of these patients, especially with good response, were excellent. Larger studies may be needed to determine the association of Dworak TRG and survival. Legal entity responsible for the study: Ramathibodi Comprehensive Cancer Center.