Nucleolus organizer regions (NORs) of the peripheral blood lymphocytes (PBL) of 9 mammalian species were analyzed by means of a silver-staining procedure. Species-specific NOR patterns were demonstrated. The number of NOR chromosomes was positively correlated with the nucleolar coefficient, and negatively correlated with the relative frequencies of uninucleolar cells in PBL interphase.
Polyhedra were solubilized under mild alkaline conditions and their structure during solubilization was studied by transmission and scanning electron microscopy. Some new structural features such as the extent of virion-packing in a polyhedral body and the cavities remaining in polyhedral bodies following the release of the occluded particles are demonstrated. Some other, previously reported features, such as the presence of polyhedral "membrane" and the crystalline array of the soluble polyhedral protein are accentuated at the various stages of solubilization.
Early reactions of rRNA-cistrons in lectin-stimulated PBL cultures of domestic pigs are shown. Interphases showing 3 or more clusters of stained areas were attributed mainly to cells after first division. Clear differences are shown between PHA- and ConA-induced NOR-expression patterns compared to the patterns in PW-stimulated cultures.
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