The use of inorganic fertilizers and pesticides can trigger the presence of heavy metals that can pollute agricultural land. The purpose of this study was to determine the bioavailability of Pb and Cu metals in cayenne pepper farms in the Subak Sembung plantation in Denpasar as well as the bioaccumulation of Pb and Cu metals in each part of the cayenne pepper plant. The method used to determine the fractions and bioavailability of Pb and Cu was a squential extraction method, while the bioaccumulation was by the destruction method quantified using the Atomic Absorption Spectrometry (AAS). The results showed that the agricultural land in Subak Sembung Denpasar both before planting and at harvesting contained Pb and Cu metals of 103.2-188.4 mg/kg and 38.3-59.3 mg/kg, respectively. The bioavailability of Pb and Cu in soil was dominated by potentially bioavailable metals (49.67-88.56% for Pb and 51.49-64.18% for Cu), then non-bioavailable (8.40-18.67% for Pb and 33.65-46.94% for Cu) and the smallest was bioavailable (1.95-12.40% for Pb and 0.41-3.31% for Cu). Metal content distributed to each part of the plants, such as Pb in the roots, stems and fruits ranged from 3.05-12.61%, 15.41-16.49%, 41.73-47.91% and 30.26-35.25%, respectively, and for Cu ranged from 42.87-47.91%, 19.13-33.76%, 17.98-26.12%, 4.54-6.84%, respectively. The edible part of cayenne pepper is classified as contaminated with Pb because it exceeds the value set by the Food and Drug Supervisory Agency, which is 0.2 mg/kg. Keywords: Bioavailability, Cu, Pb, cayenne pepper.

Contamination of heavy metals on agricultural soil can have an impact on the quality of agricultural crops. This study aimed to determine the effect of the addition of cow manure on the bioavailability of heavy metals, Cu and Pb in agricultural soil and the content of those metals in spinach plants produced from the soil. The metals contents were obtained through the sequential extraction and wet digestion methods, followed by the measurement of metal concentration using an AAS tool (Shimadzu A 7000). In this study, it was found that the metal content of Cu and Pb in spinach plants whose soil was given fertilizer of cow manure was lower than that of the soil without fertilizer. The average content of Cu and Pb in all parts of the spinach plant without fertilizer application was 53.5985 ± 0.3943 and 59.7982 ± 2.8389 mg/kg, while those given the fertilizer were 41.7626 ± 1.3590 and 52.4335 ± 0.8434 mg/kg, respectively. The level of Cu and Pb bioavailabilities also decreased with the addition of the fertilizer. The number of Cu and Pb metals being bioavailable have decreased, while the metals which were potentially bioavailable have increased significantly. Therefore, the application of cow manure as fertilizer to the agricultural soil could reduce the bioavailability of Cu and Pb metals, and their accumulation in spinach plants, as well.