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The Mars robotic sample return mission has been a potential flagship mission for NASA's science mission directorate for decades. The Mars Exploration Program and the planetary science decadal survey have highlighted both the science return of the Mars Sample Return mission, but also the need for risk reduction through technology development. One of the critical elements of the MSR mission is the Mars Ascent Vehicle, which must launch the sample from the surface of Mars and place it into low Mars orbit. The MAV has significant challenges to overcome due to the Martian environments and the Entry Descent and Landing system constraints. Launch vehicles typically have a relatively low success probability for early flights, and a thorough system level validation is warranted. The MAV flight environments are challenging and in some cases impossible to replicate terrestrially. The expected MAV environments have been evaluated and a first look of potential system test options has been explored. The terrestrial flight requirements and potential validation options are presented herein .1, 2 TABLE OF CONTENTS 1. INTRODUCTION .