Harmonization of satellite imagery provides a good opportunity for studying land surface temperature (LST) as well as the urban heat island effect. However, it is challenging to use the harmonized data for the study of LST due to the systematic bias between the LSTs from different satellites, which is highly influenced by sensor differences and the compatibility of LST retrieval algorithms. To fill this research gap, this study proposes the comparison of different LST images retrieved from various satellites that focus on Hong Kong, China, by applying diverse retrieval algorithms. LST images generated from Landsat-8 using the mono-window algorithm (MWAL8) and split-window algorithm (SWAL8) would be compared with the LST estimations from Sentinel-3 SLSTR and Himawari-8 using the split-window algorithm (SWAS3 and SWAH8). Intercomparison will also be performed through segregated groups of different land use classes both during the daytime and nighttime. Results indicate that there is a significant difference among the quantitative distribution of the LST data generated from these three satellites, with average bias of up to −1.80 K when SWAH8 was compared with MWAL8, despite having similar spatial patterns of the LST images. The findings also suggest that retrieval algorithms and the dominant land use class in the study area would affect the accuracy of image-fusion techniques. The results from the day and nighttime comparisons revealed that there is a significant difference between day and nighttime LSTs, with nighttime LSTs from different satellite sensors more consistent than the daytime LSTs. This emphasizes the need to incorporate as much night-time LST data as available when predicting or optimizing fine-scale LSTs in the nighttime, so as to minimize the bias. The framework designed by this study will serve as a guideline towards efficient spatial optimization and harmonized use of LSTs when utilizing different satellite images associated with an array of land covers and at different times of the day.