Article informationBackground: Infections caused by Extended Spectrum Beta Lactamase-producing wound pathogens are difficult to be eradicated worldwide. The prevalence rates were reported as worldwide rising, Therefore, Imperative work is needed to detect safe, non-toxic and new-effective Therapy. The Aim of The Work:To detect the first gene was isolated from female patient called temonira-1 gene, bearing Extended Spectrum beta Lactamase-producing strains among multi-drug resistant bacteria isolated from patients with wound infections. And to evaluate the antibacterial effects of PROBIOTICS' culture extracts on these isolates.Patients and Methods: Overall, 320 patients selected from those with wound infections seen in Egypt, The Wound swab samples were collected, transported in brain heart infusion broth, isolated in pure culture and bacterial species were identified by the conventional microbiological methods. Antimicrobial susceptibility testing were done, followed by Phenotypic Detection of Extended Spectrum Beta Lactamase production using the double disk synergy test, PCR technique was done to detect isolates bearing the gene. The detected isolates had tested by the PROBIOTICS' culture extracts, using the well-agar diffusion method and the reducing colony count activity technique.Results: [48.54%] of the total multi-drug resistant pathogens identified were positive for ESβL by double disk synergy test. Among 100 ESβL-producers screened, 32 isolates were detected bearing TEM-1 gene that coding for these enzymes by using PCR technique. All of the studied ESβL producers, bearing TEM-1 gene were susceptible to PROBIOTICS' CULTURE EXTRACTS with inhibition zones diameter average inbetween 12 and 21 mm., in addition, [65.6% of strains] showing reducing colony count activity by 100 % [zero CFU]. Conclusion: PROBIOTICS' CULTURE EXTRACTS revealed excellent activity againstESβL-producing microorganisms that bearing TEM-1 gene. So, these extracts may be considered safe therapeutic promising options to treat such infections.