The aim of this study was to identify immunomodulatory, apoptosis inducing and anticancer activities of aqueous and alcoholic extracts of T. purpurea, regarding the size of tumor mass and immunohistochemical detection of caspase 8 expressions and apoptotic index in mice inoculated with HepG2, mouse hepatocellular carcinoma as therapeutic experimental system for human. Forty-eight Balb / C albino mice were injected at legs with HepG2 cells. Tumor size was measured twice a week, note that the measurement time period is one month. Administration of aqueous extract (0.3,0.6,1.2) mg / kg and alcoholic extract of in (0.25,0.5,1) mg/kg to HepG2 tumor-bearing mice showed a significant difference (P≤0.01) in tumor size compared with control group. Inhibitory activity of aqueous and alcoholic extracts was dose and duration dependent. The effective doses in reduction of tumor size were 1.2 mg / kg for aqueous and 1mg/kg for alcoholic extract in which HepG2 tumor mass reduced in size for up to 87.9 %. HepG2 bearing mice treated with T. purpurea aqueous and alcoholic extracts showed significant increase (P ≤ 0.05) in apoptotic index compared with untreated control group. Extracts of T. purpurea were highly efficient for tumor growth inhibition, causing reduction in the tumor size, and increasing the expression of caspase 8 gene product in tumor tissue. Also,

This research was designed to study the effect of alcohol and alkaloids extract of Punica granatum in vivo on Lymphoid cell division of human blood in the equatorial phase also this study test toxic extracts in lymphatic cell in human used concentration 125 ,250, 500, 1000, 2000, 4000 g\ml and compared with colchicine.The results showed that alcohol and alkaloids relatively have acute toxic increased 91% in 4000 g\ml concentration with colchicien and. This study aimed to investigate the effect of the alcohol and crude alkaloids extracted from Punica granatum the on two malignant cell lines Hella and RD. Also this study included evaluation of the effect of these extracts on several cytogenetic parameters .The cytotoxicity of the alcohol extract and crude alkaloids extract was investigated on the cancer cells line, Hella and RD. Toxic effect for both extracts was indicated by rate of proliferation inhibition.The alkaloids extract showed the inhibition of Hella cell line at percentage 18.61-75.08% more than the alcohol extract 7.87-50.50% at concentrations: 62.5-4000 g/ml. The rate inhibition of alkaloids extract for RD cell line 10.48.-67.75 was higher than that of the alcohol extract 9.98-40.15 at the concentrations 62.5 and 4000g/ml.