The complete mineralization of crude oil into carbon dioxide, water, inorganic compounds and cellular constituents can be carried out as part of a bioremediation strategy. This involves the transformation of complex organic contaminants into simpler organic compounds by microbial communities, mainly bacteria. A crude oil sample and an oil sludge sample were obtained from Saudi ARAMCO Oil Company and investigated to identify the microbial communities present using PCR-based culture-independent techniques. In total, analysis of 177 clones yielded 30 distinct bacterial sequences. Clone library analysis of the oil sample was found to contain Bacillus, Clostridia and Gammaproteobacteria species while the sludge sample revealed the presence of members of the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Clostridia, Spingobacteria and Flavobacteria. The dominant bacterial class identified in oil and sludge samples was found to be Bacilli and Flavobacteria, respectively. Phylogenetic analysis showed that the dominant bacterium in the oil sample has the closest sequence identity to Enterococcus aquimarinus and the dominant bacterium in the sludge sample is most closely related to the uncultured Bacteroidetes bacterium designated AH.KK.

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The increase of anthropogenic activities has led to the pollution of the environment by heavy metals, including chromium (Cr). There are two common oxidative states of Cr that can be found in industrial effluents the trivalent chromium Cr(III) and the hexavalent chromium Cr(VI). While the hexavalent chromium Cr(VI) is highly toxic and can trigger serious human health issues, its reduced form, the trivalent chromium Cr(III), is less toxic and insoluble. Leather tanning is an important industry in many developing countries and serves as a major source of Cr(VI) contamination. Globally, tannery factories generate approximately 40 million m3 of Cr-containing wastewater annually. While the physico-chemical treatments of tannery wastewater are not safe, produce toxic chemicals and require large amounts of chemical inputs, bioremediation using chromium-resistant bacteria (CRB) is safer, efficient and does not produce toxic intermediates. Chromium-resistant bacteria (CRB) utilise three mechanisms for Cr(VI) removal: biotransformation, biosorption and bioaccumulation. This review will evaluate the three Cr(VI) detoxification mechanisms used by bacteria, their limitations and assess their applications for large-scale remediation of Cr(VI). This can be helpful for understanding the nature of Cr(VI) remediation mechanisms used by bacteria, therefore, bridging the gap between laboratory findings and industrial application of microorganisms for Cr(VI) removal.

This environmental engineering study was conducted to investigate and correlate the fundamental mineralogical compositions of phosphate rock in the Hazm Al-Jalamid area of Saudi Arabia at different layers originating from an open-pit mine. Microbial communities within these layers and the associated correlations between the mineralogical compositions at different geological and textural profiles were investigated. Three representative phosphaterock layers, upper, middle, and lower, were investigated. The dominated crystal structure of the upper layer was calcite, while dolomite, and quartz were in middle and lower layers, respectively. In contrast, Phosphate concentrations reached levels around 3.12%, 7.66%, and 3.92% of the overall content of representative samples in the upper, middle, and lower layers, respectively. The same trends were observed for both fluorapatite and phosphorus pentoxide. The microbiome of the phosphate-ore samples was evaluated. The phylum Proteobacteria dominated the upper and middle layers, while the well-known phosphate-solubilizing bacteria species Pseudomonas putida were present at 46% and 50% in both the upper and middle layers, respectively. In contrast, the actinobacterium Saccharopolyspora flava dominated the lower layer. Increasing selective phyla and species that associated with the elevated content of phosphorus from one layer to another within mine enhanced the understating of these microorganisms as extremophiles.

Al Wahbah Crater, located in a remote area in western Saudi Arabia as part of The Harrat extinct volcanic chain, is 2 km wide with a depth of 250 m. It is registered by the General Commission for Tourism and National Heritage as an ancient and archaeological site. The crater is subjected to extreme environmental conditions as its bottom is rarely subjected to rainfall and mudflows. Because of high temperature, high evaporation rates and extremely limited rainfall, the crater leaves behind dried thick white sodium phosphate crystals. Here, we studied the chemical composition and the microbial community composition using 16S rRNA pyrosequencing in different vertical layers (2, 20, 40, 60, 80 and 100 cm) of the crater sediment. Total sodium concentrations were 28 000-46 700 ppm and calcium levels were 31 400-56 500 ppm. In addition, samples were very sulphuric, with sulphate and sulphite levels exceeding 2157 ppm and 5.54 ppm, respectively. Ferric ions concentrations were <0.2 ppm, while nitrate, ammonium and nitrite levels were <2 ppm, 1.5 ppm and 0.05 ppm, respectively. Archaea dominated the surface and the bottom, while bacteria were most common at 20-60 cm. Extremely halophilic archaea and bacteria including Halorhabdus spp. Halorubrum spp., Salinibacter iranicus and Halorhodospira halophila were identified in all samples. Moreover, the relative abundance of Halanaerobiaceae accounted for 22% of the species in the top of the crater. S. iranicus and species belonging to Halorhabdus and Halorubrum that were identified between 60 and 100 cm could be considered as extreme organisms.