Hepatitis B virus (HBV) is a sexually transmitted virus with a wide range of terminal complications. As such, female sex workers (FSWs) are an important group in the epidemiology of the virus. This study was aimed at evaluating the seroprevalence of HBV markers and the exposure rate of the virus among FSWs in Enugu State, Nigeria. A cross-sectional study was carried out among brothel-based FSWs, involving 200 participants recruited using a consecutive sampling method. Blood specimens were collected and tested for HBV markers using chromatographic immunoassay rapid test kits. Additional information was obtained through the administration of a well-structured pre-tested questionnaire. Data were entered into Statistical Package for Social Sciences (SPSS) version 20.0 and analyzed using the Descriptive Statistics and Chi-Square test in SPSS. Out of the 200 sampled individuals, 82(41%) tested positive for at least one seromarker, with 44(22%) showing evidence of natural infection and 38(19%) indicating a vaccine response. Hepatitis B core antibody (total anti-HBc) was present in 42(21%) of the participants, while 8(4%) had hepatitis B surface antigen (HBsAg), which is indicative of current infection. This study revealed intermediate prevalence, a high exposure rate and a low vaccination rate among the study population. There is a need for more effective intervention strategies among FSWs in the study area.