Transparent DNA/chitosan complex film was prepared from DNA/chitosan complex powder via hydrothermal hot pressing. In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA/ chitosan complex film to determine whether this film has potential for use in membranes for bone tissue engineering. The binding ratio of DH to DNA/chitosan complex film was very close to the excision number of 3.7 for the binding of DH by native DNA. This indicated that DNA in the complex did not denature as a result of hot pressing. Apatite was formed on the surface of the film after immersion in simulated body fluid. However, quartz crystal microbalance analysis showed that protein absorption to the DNA/chitosan complex was very low. It was found that this film has an affinity for minerals rather than proteins. Although the film remained in an almost unchanged configuration at 21 days after subcutaneous implantation, the response of rat soft tissues to the film was mild. These results suggest that DNA/chitosan complex film has potential for use as a membrane in bone tissue engineering.

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At present, clinical diagnosis of maxillofacial deformity is conducted by each institution independently and a unified classification method has not been found. Therefore, it was attempted to diagnose 26 patients who were randomly chosen using their front-facial and profile pictures from five separate institutions. It was suggested that subjective factors at each institution be included to evaluate facial form classification. Our facial form classification chart was prepared to standardize clinical diagnosis and reevaluated so that the proposed classification system could be incorporated into the clinical diagnosis method. It is considered that the use of this chart would enable simple diagnosis of facial form without complicated analysis. The tentative plan of a facial form classification chart and facial form classification applied to the selected patients was presented. Comments regarding the proposal are solicited.

Abstract:To estimate whether the stored autologous blood volume of 600ml for simultaneous bimaxillary orthognathic surgery is reasonable or not, 23 patients who underwent this surgery from October 1993 to January 1995 were studied, and the following results were obtained.