Community service have the title Training and Accompaniment Propagation of  Butterfly Feed Plants for the Nimbokrang Community Jayapura Regency was carried out to provide benefits to the Nimbokrang community of Jayapura Regency who wished to develop their area into a natural tourist destination. The purpose of this service is to provide education, training and assistance in the multiplication of butterfly feed plant seedlings so that the Rhepang Village Nimbokrang community has the skills to make butterfly feed plant seedlings. The benefits of breeding skills of butterfly feed plants can be used to develop the area that wants the area to make a natural tourist destination. Planting a butterfly feed plant, which is mostly a kind of colorful flower plants, can attract the arrival of butterflies in the area so that it can add to the beauty of attractiveness and add to the vibrant natural tourism area. The dedication method used was education, training and mentoring that provided the skills for making butterfly feed plant seedings for the people of Rhepang Village Nimbokrang and assistance to provide concrete examples of how to make butterfly feed plant seedinds in the field. As a result of this dedication activity, the people of Rhepang Village Nimbokrang gained knowledge and skills in how to multiply butterfly feed plants. The scientific output of the community service was published in one of the service journals, the namely is the Papua Service Journal.Keywords: Nursery, butterfly feed plants, community skills and knoledge, Rhepang Village Nimbokrang, the beauty of natural tourism

The genetic diversity of typical clinical isolated Plasmodium falciparum in the malaria population varies greatly, especially at the location where malaria disease were recorded at high incidence rate. MSP2 is known as glycoprotein expressed on the surface of merozoites, which is an antigenic protein and has a potential to act as vaccine candidate for malaria. The MSP2 gene has two main allelic groups called FC27 and 3D7/IC. Block 3 from MSP2 gene is the most polymorphic to describe the diversity of parasite populations. The P. falciparum parasite population is often characterized by wide genetic diversity in areas of high transmission intensity. Therefore, the study on P. falciparum diversity is useful to describe the level of malaria transmission. The study of genetic diversity focused on clinical isolated species at Wamena General Hospital was aimed to determine the presence of the MSP2 gene, variety of MSP2 gene allele  and the dominant frequency of the MSP2 gene allele. This research has been carried out from March 2018 to February 2019 using a cross sectional approach. The research sample was taken and prepared from Wamena Regional Hospital and followed by the analyzing of DNA isolation, PCR, electrophoresis of the research samples was done at the genetic science laboratory in Jakarta, Indonesia. The samples studied were patients who met the inclusion criteria, namely a single P. falciparum infection with an asexual parasite density >1000 parasites/µl or >3+ (1-10 P/Lp), and were agreed to become respondents by signing an informed consent. A total of 26 clinical isolates of P. falciparum were isolated with the MSP2 gene distribution on the FC27 allele with the highest as many as 25 samples (96.2%), 22 samples (84.6%) of the 3D7 / IC allele while the mixture of the two alleles was 22 samples (84.6%). From a total of 26 samples, there were samples with the male gender category counted for 77.3% and female 41%. The results of the identification of clinical isolated P. falciparum at Wamena Hospital with a total of 26 samples were found in productive age, between 15-34 years with a single allele (95.8%), while 23 cases and mix (both alleles 87.5%) about 21 cases, meanwhile in cases of before-productive age, in which ages were 12 and 14 years of age with a single allele 100% (FC27) 2 cases and 50% (3D7/IC) found to be 1 case, The mixture of the two alleles is 50% was only 1 case and there was no sample at non-productive age observed. Key words: Malaria; MSP-2; P. falciparum; Wamena

Malaria is one of the major threats concerning world public health especially in the tropical countries, where bear the major burden of the disease, such as in Papua. Malaria was transmitted by Anopheles mosquito as malaria vector. This research aimed to determine and identify the malaria vector in Keerom Regency. Vector status was determined by the value of vector capacity or sporozoite content in Anopheles mosquito. Mosquitoes composition in the location of study were An. koliensis, An. farauti, An. punctulatus, An. subpictus dan An. brancroftii. An. subpictus and An. brancroftii were only found in a few number of individual so they were not included in vectorial capacity analysis. The result of vector capacity analysis showed that An. koliensis range from 6% to 17%, An. farauti range from 0.3% to 3%, and An. punctulatus range from 3% to 5%. Sporozoite detection using VecTOR TM Test showed that sporozoite was not found in the mosquitoes being studied. The potential malaria vectors in Keerom regency were An. koliensis, An. punctulatus and An. farauti. AbstrakMalaria merupakan salah satu masalah kesehatan utama di beberapa wilayah didunia terutama di wilayah tropis seperti halnya di Papua. Malaria disebabkan oleh nyamuk Anopeles sebagai vektor malaria. Tujuan penelitian ini adalah menentukan dan mengidentifikasikan kemampuan nyamuk Anopheles menularkan penyakit malaria di Kabupaten Keerom. Status vektor ditentukan berdasarkan kapasitas vektorial atau pendeteksian kandungan sporozoit pada nyamuk Anopheles. Komposisi nyamuk yang ditemukan di lokasi penelitian adalah An. koliensis, An. farauti, An. punctulatus, An. subpictus dan An. brancroftii. An. subpictus dan An. brancroftii hanya ditemukan dalam jumlah yang kecil sehingga tidak dilakukan analisis kapasitas vektorial. Perhitungan kapasitas vektorial menunjukkan bahwa kapasitas vektorial An. koliensis berkisar dari 6% dan 17%, An. farauti antara 0,3% dan 3%, dan An. punctulatus berkisar antara 3% dan 5%. Deteksi kandungan sporozoit menggunakan Test VecTOR TM menunjukkan tidak ditemukannya sporozoit pada nyamuk yang diteliti. Potensi nyamuk yang diduga merupakan vektor malaria di Kabupaten Keerom adalah An. koliensis, An. punctulatus dan An. farauti.

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Sibling species identification is very important in the understanding of malaria epidemiology. Morphological criteria are usually used in the identification of anopheline species, but this fails when sibling or cryptic species occur. Analysis by PCR-RFLP of rDNA ITS2 is currently the most reliable and sensitive method for distinguishing between members of the Anopheles punctulatus group. The objective of this study was to investigate the effect of DMSO concentration on ITS2 amplification of An. farauti from the colony maintained at BATAN Jakarta using PCR-RFLP based on the rDNA ITS2. The results showed that the addition of 6 % and 7 % DMSO produced ITS2 amplification products in the size 750 bp. DMSO could be used in PCR to relieve secondary structures when amplifying high GC templates. Molecular identification of An. farauti is found to be Anopheles farauti sensu stricto.Key words: molecular identification, sibling species, PCR-RFLP, rDNA ITS2, secondary structure ABSTRAK Identifikasi spesies sibling sangat penting untuk mempelajari epidemiologi malaria. Ciri morfologi secara umum digunakan dalam identifikasi spesies Anopheles, namun tidak sesuai ketika digunakan untuk spesies sibling dan spesies kriptik. Analisis dengan menggunakan PCR-RFLP dari ITS2 rDNA pada saat ini merupakan metode yang paling cocok dan sensitif untuk membedakan spesies Anopheles punctulatus group. Tujuan penelitian ini adalah untuk mengetahui pengaruh konsentrasi DMSO terhadap amplifikasi ITS2 dalam mengidentifikasi An. farauti dari koloni di BATAN Jakarta dengan PCR-RFLP berdasarkan ITS2 rDNA. Hasil penelitian menunjukkan bahwa penambahan DMSO dengan konsentrasi 6% dan 7% menghasilkan produk amplifikasi ITS2 dengan ukuran 750 pb. DMSO dapat digunakan dalam PCR untuk mencegah terbentuknya struktur sekunder *Penulis korespondensi: Ign.