Chymosin has been identified in rennet samples from stomaches of living calfs by means of it's isoelectric points (IP), molecular weight (Mol. Wt.) and enzymatic activity.The values obtained by isoelectric focusing (IEF) and a two-dimensional electrophoretic technique, amount 4,5...4,7 (IP) and 36300 (Mol. Wt.) respectively. Furthermore the fractionation of calf rennet by IEF has been performed in a preparative scale. From one gramme of calf rennet we obtained 53 mg of purified chymosin, which shall be used for preparing antisera against chymosin
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