The most commonly used method to control insect pests, such as leaf-cutting ants (LCA) in forest plantations, is the application of chemical insecticides, but their use was forbidden in plantations certified by the Forest Stewardship Council because of their negative effect on the environment. 2 A 5-year derogation of their prohibition was obtained in 2016 in Argentina, on the condition that forestry companies reduce pesticide doses by 5% per year and inform the Economic Injury Level (EIL). 3 The EIL and the Economic Threshold (ET) were estimated for the first time for the Acromyrmex genus in a Salicaceae forestation. As a model, we used the damage caused by the most detrimental LCA species of Argentina (Acromyrmex lundii) in the first, most critical year of a willow plantation (Salix nigra) in the lower basin of the Paraná River. 4 The EIL (8-15 nest/ha) was useful to demonstrate the real magnitude of the damage caused by A. lundii, which justified the need for its control. The ET for profitable production (∼3 nests/ha) would not be operatively applicable in a long-term management program in our study site. This methodology could be extrapolated to other Neotropical forest plantations attacked by Acromyrmex species.