The study was conducted to determine the effect of Dayak onion (Eleutherine palmifolia) extract combined with Lactobacillus acidophilus on intestinal lactic acid bacteria (LAB) and coliform in broiler. In vitro study (first experiment) was assigned in a completely randomized design with 3 x 3 factorial scheme. Levels of Dayak onion extract (first factor) were 25% (D1), 50% (D2) and 75% (D3), and levels of Lactobacillus acidophilus (second factor) were 106 cfu/mL (L1), 107 cfu/mL (L2) and 108 cfu/mL (L3). Parameters measured were total phenolic, flavonoid and antioxidant activity. To investigate lactic acid bacteria (LAB) and coliform, in vivo study (second experiment) was conducted using 192 birds of one-day old broiler and were divided into 4 treatments (6 replications, 8 birds each). Dietary treatments were basal diet without combination of Dayak onion extract and Lactobacillus acidophilus (EpLa0), basal diet added with 0.1% EpLa (EpLa1), 0.2% EpLa (EpLa2) and 0.3% EpLa (EpLa3). Data were subjected to analysis of variance and Duncan multiple range test (p<0.05). Increasing levels of Ep extract combined with La significantly (p<0.05) increased phenolic and flavonoid contents, and improved antioxidant activity. Intestinal LAB growth significantly enhanced (p<0.01), while coliform counts significantly decreased (p<0.05) with the increasing level of feeding EpLa. In conclusion, feeding combination of Dayak onion extract and Lactobacillus acidophilus effectively increase antioxidant capacity and improve digestive tract condition based on the higher LAB and lower coliform populations in broiler.

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Objective: The feeding effects of DoLa (a combination of Dayak onion extract and probiotic Lactobacillus acidophilus ) on hematological indices and lymphoid organs as indicators of broiler health status were evaluated in the present study. Materials and Methods: 192 1-day-old unsexed broilers of the CP 707 strain with a body weight of 46.43 ± 1.65 gm were randomly divided into 4 dietary treatments with 6 replications. The dietary treatments applied were basal diet (BD) as a control with a code of DoLa0, BD + 0.1% DoLa (DoLa1), BD + 0.2% DoLa (DoLa2), and BD + 0.3% DoLa (DoLa3). The parameters monitored included hemoglobin (Hb), red blood cell (RBC), heterophile (H), lymphocyte (L), white blood cell (WBC), heterophile-lymphocyte (H/L) ratio, the lymphoid organs (bursa Fabricius, spleen, and thymus) relative weight, as well as carcass weight. Results: The results indicated a significant improvement in WBC, L, and carcass weight ( p < 0.05) as the feeding level of DoLa increased while the H and H/L ratio decreased. However, the dietary inclusion of DoLa did not affect the lymphoid organs’ relative weight, RBC, and Hb concentrations. Conclusion: The mixture at 0.3% significantly improved health status through the indicators of hematological indices, lymphoid organs, and carcass weight of broilers.