Latar Belakang. Lesi melanositik memiliki gambaran morfologi dan sitomorfologi yang luas mencakup lesi jinak yaitu nevus melanositik dan lesi ganas yaitu melanoma maligna. Beberapa gambaran nevus melanositik menyerupai melanoma. p15 merupakan gen penekan tumor dan biomarker yang kuat sehingga dapat digunakan untuk membedakan antara nevus dan melanoma. Tujuan. Mengetahui hubungan tingkat ekspresi p15 pada nevus dan melanoma. Metodologi. Penelitian ini merupakan penelitian deskriptif observasional dengan desain serial kasus sejak 1 Januari 2015 sampai 30 Desember 2019. Sebanyak 60 sampel dilakukan pemeriksaan imunohistokimia menggunakan antibodi p15. Ekspresi p15 dinilai berdasarkan proporsi sel tumor yang terpulas dan intensitas pulasan. Analisis statistik menggunakan SPSS versi 23.0. Hasil. Nevus melanositik paling banyak dijumpai pada perempuan (76,7%), pada kelompok usia <55 tahun (90%), lokasi tumor pada kepala dan leher (83,3) dan pada tipe histopatologi dermal naevus (53,3%). Melanoma maligna paling banyak ditemukan pada jenis kelamin laki-laki (53,6%), pada kelompok usia <55 tahun (57,1%), lokasi tumor pada kepala dan leher (35,7%) dan pada tipe histopatologi nodular melanoma (42,9%). Terdapat hubungan bermakna antara tingkat ekspresi p15 dengan lesi melanositik (nevus melanositik dan melanoma maligna) (r=0,9666; p=0,000). Kesimpulan. Ekspresi p15 tinggi dijumpai pada nevus melanositik dan rendah pada melanoma maligna. Terdapat hubungan bermakna antara tingkat ekspresi p15 dengan lesi melanositik (nevus melanositik dan melanoma maligna). Semakin rendah ekspresi p15 maka semakin besar kemungkinan terjadi melanoma maligna.

Dermatitis herpetiformis (DH) is a rare autoimmune bullous disease characterized by intensely pruritic, chronic, and recurrent vesicles on extensor surfaces such as the elbows, knees, and buttocks. There is a genotype relationship with HLA-DR3, HLA DQw2, discovered about 80-90% of cases. Immunoflorescence is the gold standard for diagnosis, but serologic testing can help if immunofluorescence result is negative. On histopathological examination, at the tips of papillary dermis, a collection of neutrophils are found and granular immunoglobulins A. Dermatitis herpetiformis associated with gluten intolerance (celiac disease), although the mechanism is not fully understood. Patients with gluten free diet will reduce of this disease both in the skin and intestinal tract, thereby reducing risk of lymphoma progression. Dapsone is the main therapy, but it require monitoring side effects.

A B S T R A C TIntroduction: Basal cell carcinoma (KSB) is a non-melanoma skin cancer (KKNM),which is most commonly found compared to other skin cancers. KSB originates fromstem cells in the bulk of hair follicles or inter-follicular epidermis, through the Sonichadgehog (SHH) activation pathway, an increase in Sonic hadgehog (SHH) proteinexpression, involving Patches protein (PTCH), smothened protein (SMO), in the formof increased protein transcription activation Glia (GLI) in the nucleus, binds to DNAto initiate tumor-aggressive growth and tissue. Objective: to determine therelationship between Sonic hadgehog (SHH) expression and non-aggressive andaggressive basal cell carcinoma. Methods: The study was carried out in anobservational laboratory with 35 primary KSB patients, the tissue was taken usingelliptic biopsy technique, made paraffin block specimens for histopathologicalexamination of the subtype of KSB consisting of 20 non-aggressive KSB patients,namely nodular and superficial KSB; 15 patients with aggressive KSB werepigmented KSB; Infiltrates KSB, micronodular KSB, metypical KSB (basosquamousKSB) and SHH immunohistochemical (CPI) examination using SHH antibodies, inthe Anatomy Pathology section, FK Unsri / RSMH Palembang. The characteristics ofKSB patients were recorded, namely sex, age, occupation based on the length ofexposure to BC, namely exposure <3 hours / day, exposure 3-6 hours / day,exposure ≥ 6 hours / day. The data were processed using the Statistical AnalysisSoftware Package (SPSS) version 20.0 (IBM Corporation), tested with Pearsoncorrelation test and chi square test and presented in the form of diagrams, andnarrative tables. Results: Pearson's test showed a significant correlation betweenthe clinical features of KSB and the histopathologic features of non-aggressive andaggressive types of KSB (p 0.020), there was a significant relationship between thesubtypes of histopathologic features of KSB with non-aggressive and aggressivetypes of KSB (p 0.000), there was a significant relationship between strong SHHexpression and BCC aggressive compared to non-aggressive KSB, p 0.000 (p <05α),and r = 732 Conclusion: There is a relationship between SHH expression and KSBaggressiveness. The increase in strong SHH expression shows the aggressiveness ofKSB, SHH expression can be used as a biological gene target both as a prognosticindicator and can be used as a target for treatment of aggressive KSB, especially inthe elderly.

Abstract Background: Basal cell carcinoma (BCC) is a malignant tumor originating from non- keratinizing cells originating from the basal layer of the epidermis. Basal cell carcinoma is the most common malignant tumor, nearly 90% of all non-melanoma skin cancers. Clinically, BCC is difficult to distinguish, final diagnosis determined based on histopathological examination of complete tumor tissue. Based on histological growth patterns, BCC is divided into two, non- aggressive type (nodular and superficial) and aggressive type (infiltration, micronodular, fibroepithelial, basoskuamosa, keratotic, adnexal differentiation). The examination technique by analyzing the proteins or genes involved is related to the aggressiveness of basal cell carcinoma. Objective: This study was conducted to determine the comparison of expression of Bcl-2 in periocular basal type aggressive and non-aggressive carcinomas in dr. Mohammad Hoesin Palembang. Methodology: A cross sectional analytic study from 26 periocular basal cell carcinoma patients in RSUP dr. Mohammad Hoesin Palembang, January 2014- June 2018. Histopathologic features were examined with hematoxylin-eosine staining and imunohistochemical were examined with Bcl-2 monoclonal antibody. Data were analyzed by bivariate analysis, using chi-square test and fisher exact test. Results: Positive Bcl-2 expression was more common in periocular basal cell carcinoma (93.8%) than aggressive (50%), and nodular type (93.8%) compared to other types of histological differentiation. Positive Bcl-2 expression was also higher in female patients (90.9%) than males (66.7%). There is a significant relationship between expression of Bcl-2 and growth patterns, differentiation of cell histology and also sex of patients with basal cell carcinoma. Conclusion: In this study, there was a significant correlation between expression of Bcl-2 and aggressive and non-aggressive periocular basal cell carcinoma in RSUP dr. Mohammad Hoesin Palembang.

ABSTRACT Background: Retinoblastoma is a malignant tumor with an average incidence of one case per 15,000-20,000 births. Retinoblastoma is associated with the presence of the Rb1 gene mutation which has role in preventing DNA replication. Various studies have linked the existence of p16INK4A as a CDK inhibitor that inhibits Cyclin D and CDK4 so that the activity of the Rb1 gene is inhibited. Objective: This study was conducted to identify p16INK4A expression on Retinoblastoma at Mohammad Hoesin Hospital. Methodology: A descriptive study used on the paraffin blocks of 14 cases Retinoblastoma in Mohammad Hoesin Hospital Palembang from January 2014- July2018. Histopathologic feature were examined with Hematoxylin- Eosin staining and immunohistochemical were examined with p16INK4A antibody. Data were analyzed using SPSS version23 to identify histopathological feature of p16INK4A expression in Retinoblastoma Results: Most retinoblastoma samples in this study were expressed in p16INK4A (92.86%). The degree of differentiation of retinoblastoma with the largest number at Mohammad Hoesin Hospital is moderately differentiated (57.14%). Retinoblastoma invasion of the optic nerve and sclera in the study at Mohammad Hoesin Hospital, respectively at 57.14% and 42.86%. Conclusion: Most retinoblastoma samples in this study were expressed in p16INK4A.