Leptospirosis merupakan penyakit zoonosis disebabkan bakteri Leptospira patogenik dan berpotensi menyebabkan kematian pada manusia. Tikus sebagai reservoir utama Leptospira kebanyakan diteliti di kawasan perkotaan atau pedesaan di sekitar permukiman. Sebaliknya, penelitian leptospirosis pada tikus endemis jumlahnya sedikit. Penelitian ini bertujuan untuk mengidentifikasi jenis tikus endemis Sulawesi sebagai reservoir Leptospira di Kabupaten Bulukumba, Pangkep dan Luwu Timur. Penelitian ini merupakan bagian dari Rikhus Vektora tahun 2017 dengan menggunakan perangkap hidup untuk menangkap tikus di enam ekosistem berbeda pada tiap kabupaten. Seluruh tikus tertangkap diidentifikasi secara morfologis yang selanjutnya diuji Microscopic Agglutination Test (MAT) dan Polymerase Chain Reaction (PCR). Hasilnya diperoleh berbagai tikus endemis seperti Rattus marmosurus, Rattus hoffmanni, Bunomys chrysocomus, Bunomys andrewsi dan Bunomys coelestis terinfeksi leptospirosis. Hasil penelitian menunjukkan catatan baru beberapa jenis tikus endemis Sulawesi terinfeksi leptospirosis. Kondisi ini perlu diwaspadai karena terdapat potensi penularan leptospirosis di habitat hutan dari tikus endemis tersebut.

Bantul is one of leptospirosis endemic areas which has been reported the cases every year and the presence of rats is an important risk factor relating to leptospirosis incidence in Bantul. Therefore, the role of rats as reservoir was examined in three types of ecosystems: forest, non-forest, and coastal ecosystems. Rat trapping was carried out using 100 single livetraps which were distributed in 9 locations: 2 points in the forest, 5 points in the non-forest, and 2 points in the coastal ecosystem. The rats were identified and their kidneys were collected and preserved in 70% alcohol medium. Leptospira were detected in the kidney by using PCR method. A number of 196 rats were obtained during the study. Most of them were commensal rats obtained from settlements ecosystems. Rattus tanezumi was the dominant species in the three ecosystems, but the highest infection rate of leptospira pathogen was in R.norvegicus. Coastal ecosystems were contained more infected rats, where the main habitat were mangrove forests. Based on these results, rats have great potency in leptospirosis transmission in Bantul, especially in settlements and coastal areas although leptospirosis cases were reported rarely in study area. Nonetheless, awareness of transmission should be disseminated since the presence of pathogenic leptospira in rats is very high. Keywords: leptospirosis, rat, reservoir, ecosystem, Bantul Abstrak Kabupaten Bantul merupakan salah satu daerah endemis leptospirosis yang sejak lama melaporkan kasus ini setiap tahunnya dan keberadaan tikus merupakan faktor risiko penting yang mempengaruhi kejadian leptospirosis di Kabupaten Bantul. Oleh karena itu peranan tikus sebagai hewan reservoir diteliti di tiga jenis ekosistem yaitu ekosistem hutan, non-hutan, dan pesisir. Penangkapan tikus dilakukan menggunakan perangkap tunggal sebanyak 100 buah yang disebar di 9 titik lokasi, meliputi dua titik di ekosistem hutan, lima titik di ekosistem non-hutan, dan dua titik di ekosistem pesisir. Tikus tertangkap diidentifikasi kemudian organ ginjalnya dikoleksi dan diawetkan dalam medium alkohol 70%. Bakteri leptospira dideteksi pada specimen ginjal dengan metode PCR dari total 196 tikus diperoleh selama penelitian. Sebagian besar tikus diperoleh dari ekosistem dekat pemukiman dan semuanya merupakan tikus komensal. Rattus tanezumi merupakan spesies dominan di ketiga ekosistem, namun persentase infeksi leptospira patogen paling tinggi terdapat pada R. norvegicus. Ekosistem pesisir mengandung tikus terinfeksi leptospira patogenik paling tinggi, dengan habitat utama hutan mangrove. Berdasarkan hasil ini maka tikus berpotensi besar menularkan leptospirosis di Kabupaten Bantul, terutama di daerah pemukiman dan pesisir. Meskipun kasus leptospirosis di daerah penelitian sedikit sekali dilaporkan, namun kewaspadaan terhadap penularan perlu dilakukan sedini mungkin karena leptospira patogenik yang terkandung dalam tikus tergolong tinggi. Kata kunci: leptospirosis, tikus, reservoir, ekosistem, Bantul

Central Sulawesi is one of the malaria-endemic areas in Indonesia. Anopheles nigerrimus, Anopheles barbirostris, Anopheles subpictus, Anopheles parangensis, Anopheles flavirostris and Anopheles minimus were confirmed as malaria vectors. Researchers believed that there might have a distinctly different distribution and diversity of the vectors in different ecosystems. The purpose of the current study was to investigate and analyze the distribution and diversity of suspected malaria vectors (Anopheles spp.) in various ecosystems. Adult mosquitoes were collected using some methods e.g. human man landing method, animal baited trap, mosquito caught around the cage, using sweep net and the light trap. The sporozoites of Plasmodium infecting the caught mosquitoes were identified and examined using PCR. In addition, a survey on potential breeding sites of the mosquitoes. Based on the survey results, the mosquitoes species caught in the non-forest ecosystems near the settlements and positively infected by Plasmodium were An. ludlowae, An. flavirostris, and An. vagus. Meanwhile, the malaria vectors caught in the non-forest ecosystems far from the settlements were An. maculatus and An. subpictus. The results also demonstrated that An. barbirostris was detected as a malaria vector in the coastal area near the settlement. The ponds, rivers and fish farms were potential breeding sites for the mosquitoes. The suspected malaria vectors (Anopheles spp) spread throughout ecosystems e.g. forests, non-forests and coasts. The high risk of ecosystems for malaria transmission was the non-forest ecosystems near the settlements, the non-forest far away from the settlements, and the coasts near the settlements.

Leptospirosis is a disease caused by pathogenic Leptospira and is still a health problem in the community. Until now, West Papua Province has not reported any cases of leptospirosis. This study aims to examine whether there is a grouping pattern of Leptospira harboring rats and identify the habitat types of positive rat Leptospira. This research was descriptive observational research using a cross-sectional approach. Research locations are in Manokwari, Fakfak, and Raja Ampat districts in West Papua Province. The catching of rats was carried out in locations of forest ecosystem settlements, near forest settlements, non-forested far from settlements, non-forests near settlements, coastal remote settlements, and beaches near settlements. Leptospira in rats was detected by examination of Polymerase Chain Reaction (PCR) and Microscopic Agglutination Test (MAT). The results showed  278 rats were caught. The number of Leptospira positive rats in three districts by  PCR was 34 (12.2%) and MAT were 13 (4.7%). Leptospira positive habitat types were settlements, yards, and secondary forests. The SaTScan results were obtained by six positive Leptospira rat clusters. There were no significant results which showed that no spread of  Leptospira in the clusters. The presence of Leptospira in rats should be  a warning for leptospirosis transmission risk at the study site.

Macroglossus sobrinus, Cynopterus brachyotis, dan Cynopterus sphinx terkonfirmasi sebagai reservoir Lyssavirus di Provinsi Riau. Penelitian dilakukan di tiga kabupaten, yaitu Kabupaten Kepulauan Meranti, Kota Dumai, dan Kabupaten Bengkalis. Lokasi pengambilan sampel dilakukan pada ekosistem hutan, non hutan, dan pantai.Penangkapan kelelawar dilakukan menggunakan mist net, harp net, dan hand net. Kelelawar terkoleksi diambil sampel swab trakea untuk pemeriksaan Lyssavirus. Deteksi Lyssavirus dilakukan meggunakan metode PCR. Dari 356 sampel yang diperoleh, 19 sampel terkonfirmasi positif Lyssavirus.