Background and Purpose. Health Care-Associated Infections, hereinafter abbreviated as HAIs, are infections that occur in patients during treatment in hospitals and other health care facilities where when they enter there are no infections and are not in the incubation period, including infections in the hospital but appear after patients returning home, as well as infections due to work for hospital staff and health workers related to the process of health services in health care facilities. Health Care-Associated Infection (HAIs) is a serious problem for all healthcare facilities worldwide, including in Indonesia. The purpose of this study was to analyze the influence of organizational culture, knowledge and motivation simultaneously or individually on the behavior of doctors in infection prevention and control. Methods: This study uses a quantitative approach with an explanatory causality research design. The technique of collecting data was using a questionnaire and tested using Path Analysis. Results: The research shows that the above confirms Lawrence's theory that a person's behavior in carrying out an activity wherein this study is infection prevention and control measures are determined by three factors, namely Predisposing factors (self-initiated factors), Enabling factors (enabling factors) and Reinforcing factors. (reinforcing factor). Implications: This research helps hospital management develop a more structured infection prevention and control committee (PPI) program and develop a reporting system for each action in infection prevention and control based on electronic or web.