Objective: The use of ethnobiology in treatment of many diseases especially in rural residents with limited access to medical technology, treatment and equipment is beneficial and necessary. Daucus carota (DC) root extract was used as pre-treatment in adult Wistar rats exposed to diclofenac sodium (DF) to investigate a protective effect on the histology of liver, kidney and duodenum. Materials and Method: Twenty-five adult Wistar rats were used for this study and were randomly divided into 5 groups of 5 rats each, which included: Group A - Normal control; Group B – 50mg/kg DF control; Group C – 50mg/kg DC + 50mg/kg DF, Group D – 100mg/kg DC + 50mg/kg DF; and, Group E – 140mg/kg DC + 50mg/kg DC. Results and Discussion: The results showed a reduction in damage to the hepatocytes, maintenance of sinusoidal integrity and reduction in the number of inflammatory cells in the hepatic parenchyma, In the kidney tissue, the extract preserved the glomerular capillary tuft, renal tubular epithelial cells, conserved Bowman’s space and lining epithelium of the capsule in a dose dependent manner. The intestinal mucosa in groups treated with higher doses of the extract were completely preserved and intact with minimal erosion of  epithelial lining along with preservation of lamina propria and intestinal glands. Conclusion: Pre-administration of DC, preferably at concentrations of 100mg/kg and 140mg/kg reduced hepatotoxicity, renal tissue and mucosal layer damage in the duodenum following administration of DF. This preservation of tissues improved as concentration of the extract increased verifying that its efficiency was dose dependent.