Background:The main characterizations of foot deformity, which is called congenital talipes equinovarus, in severity and variation degree depend on complications and congenital malformations. They are classified into four main components such as midfoot cavus, forefoot adducts, heel/hind foot various and hind foot equines.Objective: This study aimed to estimate some of biochemical markers such as asporin, fibromodulin and tenascin-C in club foot child disease to determine pathological causes of severe deformity of bone, for monitoring complications of bone mineralization and calcification and for early treatment using drugs of choice. Patients and Methods: 60 Clubfoot patients with dislocation dysplasia of Hip (DDH) disease were included in this study. The samples were collected from Clubfoot Unit and private centers in Holy Najaf, Iraq during the period from December 2020 to February 2021. The patients groups were divided into subgroups according to age, gender, idiopathic, syndrome, Parina score, body mass index (BMI) and clubfoot. The control group included 30 apparent healthy participants and the age was nearly matched with patients' groups. Results:The level of tenascin-C was increased significantly in clubfoot patients than in control group. Also, tenascin-C level was highly increased in advanced ages (3-5) years in comparison with other ages and in males than in females. Also, high score (6-7) was significantly increased than other scores. The present results showed that club foot with dislocation dysplasia of hip was highly significant compared to clubfoot only and dislocation dysplasia of hip only in all children patients. Moreover, clubfoot syndrome was higher than idiopathic syndrome significantly whereas no differences in tenascin-C level in all subgroups according to BMI (normal, overweight and obese) of children of clubfoot. Conclusion: Increased levels of tenascin-C is very important as a pathological marker in patients of clubfoot with dysplasia of Hip.