Background Diospyros kaki L. is considered one of the most important economical medicinal plant in Chinese herbal medicine belonging to family Ebenaceae as it contains varied secondary metabolites and used in treatment of many diseases. For there is an efficient and promising protocol for in vitro propagation of Diospyros kaki L. cv. Costata was established Diospyros kaki L. cv. Costata although it is difficult to initiate it in sterilized artificial media due to the browning of tissue caused by oxidation of phenolics. An efficient sterilizing method for leaf and internode explants was obtained by using 0.2% mercuric chloride (Hg2Cl2) for 5 min. Woody plant medium (WPM) supplemented with 2 mg/l zeatin (Zt) + 5 mg/l isopentenyl adenine (2 iP). Calli were induced on Murashige and Skoog medium (MS) augmented with 10 mg/l Zt + 10 mg/l indole-3-acetic acid (IAA) + 500 mg/l polyvinylpyrolidone (PVP) + 0.1 mg/l thiamine HCL from internode explants. However, the regeneration efficiency was obtained with ½ MS-media fortified with 1 mg/l Zt + 2 mg/l IAA + 4 mg/l benzylaminopurine (BAp) + 0.5 g/l PVP from internode calli explants. Results The highest amounts of scopoletin 57.08, 26.42, and 25.30 (μg/g DW) were detected using reversed phase of high-performance liquid chromatography (RP-HPLC) in leaves extract of intact plant followed by regenerated and calli cultures of internod explants, respectively. Conclusion This study is the first record for in vitro propagation and production of secondary metabolites from Diospyros kaki L. using biotechnology techniques. Chemical anaysis were carried out using HPLC technique.

Valeriana officinalis L is the most cost-effective plant in Europe. Due to its cardiovascular, anxiolytic, sedative, and anticonvulsant properties, the root of Valeriana is extensively employed germinated in vitro by soaking for 24 hours in 150 ppm Gibberellic acid (GA3) further, cultivated in half MS (Murashige and Skoog salts medium with vitamins) medium. Treatment of MS media with 5 ppm of Benzyl a Dichlorophenoxy acetic acid (2,4-D) increased fresh and dry weights of calli produced from leaf explants. However, supplemented MS media with 3 ppm BA and 1 ppm 2,4 augmentation of MS-medium with 0.1 ppm Indole was accomplished by using peat moss:vermiculite (1:1). Valerenic acid and valtrate were analyzed qualitatively and quantitatively by HPLC in mother plant, callus, regenerated shoots, roots, and Dormival medication which used as a control. The findings showed that regenerated shoots had the greatest concentration of valerenic acid across various extracts (3.80±0.82mg/g DW), whereas in vitro-derived roots from leaf explants contained the highest concentration of valtrate (3.82±0.23mg/g DW).

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