Soil erosion is a severe and continuous environmental problem caused mainly by natural factors, which can be enhanced by anthropogenic activities. The morphological relief with relatively steep slopes, the dense drainage network, and the Mediterranean climate are some of the factors that render the Paleochora region (South Chania, Crete, Greece) particularly prone to soil erosion in cases of intense rainfall events. In this study, we aimed to assess the correlation between soil erosion rates estimated from the Revised Universal Soil Loss Equation (RUSLE) and the landscape patterns and to detect the most erosion-prone sub-basins based on an analysis of morphometric parameters, using geographic information system (GIS) and remote sensing technologies. The assessment of soil erosion rates was conducted using the RUSLE model. The landscape metrics analysis was carried out to correlate soil erosion and landscape patterns. The morphometric analysis helped us to prioritize erosion-prone areas at the sub-basin level. The estimated soil erosion rates were mapped, showing the spatial distribution of the soil loss for the study area in 2020. For instance, the landscape patterns seemed to highly impact the soil erosion rates. The morphometric parameter analysis is considered as a useful tool for delineating areas that are highly vulnerable to soil erosion. The integration of three approaches showed that there is are robust relationships between soil erosion modeling, landscape patterns, and morphometry.