Hazardous Waste Management (HWM) is critical to human health outcomes and environmental protection. A survey-based observational study was conducted in scientific laboratories of a public and a private university in Lebanon, where a total of 309 participants were recruited to evaluate the knowledge regarding safety measures to be applied when collecting and storing waste. Chi-square and Fisher's exact tests, Independent Sample T-test, Pearson correlation coefficient, and ANOVA were used for comparing differences and associations among socio-demographic variables. Linear regression models were used to map predictors of knowledge score and its relationships with demographic variables as well as training on proper HWM. A significant association (p<0.05) was found between knowledge score and job function, years 'experience, educational level, professional status, work schedule, and training on HWM. Participants had adequate perceptions regarding the impact of HWM on health and the environment. Linear regression modeling revealed that knowledge score was significantly higher among bachelor level lab workers compared to those with doctoral degrees (p=0.043), full-time workers versus part-timers (p=0.03), and among public university participants compared to those of private university (p<0.001). It highlights the importance to improve the culture, attitude, and practice of proper HWM in scientific laboratories.