Hemolytic strains of Aeromonas spp. from fish and fishery products were detected by multiplex PCR. The selected primers for the amplification of segments of ahh1, asa1 and 16S rRNA gene yielded products with the size of 130 bp, 249 bp and 356 bp, respectively. This assay was found to be highly sensitive, as it could detect 7 and 9 cells of Aeromonas hydrophila and A. sobria with a detection limit of 1 pg of pure genomic DNA. The assay, when screened for 73 commercial fish and fishery product samples consisting of freshwater, marine fish and shellfish, showed 56 % positive for Aeromonas spp., 16 % for Aeromonas hydrophila and 13 % for A . sobria. This assay provides specific and reliable results and can be a powerful tool for the simultaneous detection of hemolytic strains of A . hydrophila A . sobria and other Aeromonas spp. from fish and fishery products.

The present study was undertaken to find out the incidence of Motile Aeromonas Septicaemia (MAS) in the freshwater aquaculture system in three districts viz. Nagaon, Morigaon and Sonitpur of Assam. A total of 293 number pools were screened for bacteriological and mycotic studies. Out of 293 pools screened, 91 (31.05%) were found to be positive for Aeromonas species, 131 (44.70%) for A. invadans and 55 (18.77%) for S. parasitica. Among Aeromonas genera, A. hydrophila, A. veronii and A. sobria were detected with 51.64%, 21.97% and 18.68% respectively. The prevalence of other Aeromonas species (7.69%) was also recorded during the study. Data on the seasonal prevalence of MAS reveal that the highest prevalence (62.63%) was recorded during the winter season followed by post monsoon (20.87%) and premonsoon (12.08%). Thus, study suggests that motile aeromonads remain an important bacterial pathogen from aquaculture point of view in Assam, which requires regular and constant examination of cultured fish to resist unexpected losses.

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This paper uses annual accounting data to show that the frequency of occurrence of the first and second digits contained in the income numbers of companies, listed in the Istanbul Stock Exchange, does not deviate significantly from expectations.

A total of 103 representative colonies from 53 healthy and diseased food fish samples were tested for the incidence of Aeromonas spp. Of which 57% of colonies were positive for Aeromonas spp. by the multiplex PCR (MPCR). On an average, the prevalence was found to be 66% with highest incidence in diseased fishes (76%). About 48% of healthy fish samples were observed to contain Aeromonas. The biomoleculoar identification revealed that the selected virulent hemolytic and enterotoxigenic genes appeared in four different patterns viz. alt; act//hlyA/aer; alt and act//hlyA/aer; ast, alt and act//hlyA/aer, among the MPCR positive isolates of Aeromonas spp. The overall prevalence of the toxin genes alt, act/hlyA/aer and ast among the MPCR positive Aeromonas isolates was found to be 89.8%, 72.9% and 20%, respectively. The biochemical confirmation of the MPCR positive Aeromonas isolates revealed different species in the tested samples belonging to A. hydrophila, A. sobria, A. caviae, A. jandaei, A. veronii, A. schubertii and A. trota and the dominant species was found to be A. hydrophila (48%).