Introduction: Anemia in pregnant women is also said to be potentially harmful not only to the mother but also to the child she is carrying. The impact that will occur from anemia is the higher risk of miscarriage, premature birth, low birth weight, disorders that occur in the fetus and can increase maternal and fetal morbidity and mortality Objectives: The purpose of this study was to determine the relationship between the role of the midwife, the role of the family, and the mother's perception of the behavior of preventing anemia in pregnant women in the Lawang Gintung Public Health Center in 2021 Method: The method used in this study was a descriptive quantitative and analytical approach using a cross-sectional design. The population of this study was 149 pregnant women and the sample used was 65 pregnant women as respondents. The sampling technique is simple random sampling. The measuring instrument used is a questionnaire. The analytical method used is Chi-Square with 0.05 using IBM spss v25 Result: The results showed that anemia prevention behavior in pregnant women was included in the good category (50.8%), the role of the midwife was supportive (72.3%), the role of the family good (63.1%), good mother perception (53.8%) in anemia prevention behavior in pregnant women in the Lawang Gintung Health Center area in 2021. Conclusion: In conclusion, there is a significant relationship between the role of the midwife (P-value = 0.044), the role of the family (P-value = 0.016), and the  mother's perception (P-value = 0.004) of anemia prevention behavior in pregnant women in the Lawang Gintung Public Health Center area in 2021. Mother's perception is the variable most related to anemia prevention behavior in pregnant women. The better the mother's perception, the better the anemia prevention behavior.