BackgroundIonizing-radiation-resistant bacteria (IRRB) show a surprising capacity for adaptation to ionizing radiation and desiccation. Positive Darwinian selection is expected to play an important role in this trait, but no data are currently available regarding the role of positive adaptive selection in resistance to ionizing-radiation and tolerance of desiccation. We analyzed the four known genome sequences of IRRB (Deinococcus geothermalis, Deinococcus radiodurans, Kineococcus radiotolerans, and Rubrobacter xylanophilus) to determine the role of positive Darwinian selection in the evolution of resistance to ionizing radiation and tolerance of desiccation.ResultsWe used the programs MultiParanoid and DnaSP to deduce the sets of orthologs that potentially evolved due to positive Darwinian selection in IRRB. We find that positive selection targets 689 ortholog sets of IRRB. Among these, 58 ortholog sets are absent in ionizing-radiation-sensitive bacteria (IRSB: Escherichia coli and Thermus thermophilus). The most striking finding is that all basal DNA repair genes in IRRB, unlike many of their orthologs in IRSB, are subject to positive selection.ConclusionOur results provide the first in silico prediction of positively selected genes with potential roles in the molecular basis of resistance to γ-radiation and tolerance of desiccation in IRRB. Identification of these genes provides a basis for future experimental work aimed at understanding the metabolic networks in which they participate.

This study evaluates the influence of various doses of gamma irradiation on some quality aspects of iced bonito (Sarda sarda). Fresh bonito sections were aerobically packed and gamma irradiated with doses of 0, 1.5, 3, 4.5, 6, and 7.5 kGy, samples were then chilled during 21 days. Microbiological analyses showed the presence of mesophilic and psychrophilic germs just before irradiation treatment. These germs were eliminated at doses !1.5 kGy. The analyses of total volatile basic nitrogen (TVB-N), trimethylamine (TMA) as well as the texture analysis showed that irradiated samples preserved a good quality during 21 days of chilled storage, whereas the control showed putrefaction. Histamine content was reduced after irradiation treatment, such decrease strongly correlated with the applied dose level. Fatty acid composition survey demonstrated that gamma irradiation associated with aerobic packaging influenced significantly the lipid profile. Irradiation at doses of 6 and 7.5 kGy reduced significantly poly-unsaturated fatty acid fraction. Results demonstrated that irradiation amplified the rate of lipid oxidation as shown by an increased peroxide value especially at higher doses. Gamma irradiation at low doses was effective in extending the bonito's shelf-life through the improvement of its microbiological, biochemical and textural qualities up to 21 days of chilled storage.

The purpose of the present research is to study the production of thermophilic alkaline protease by a local isolate, Streptomyces sp. CN902, under solid state fermentation (SSF). Optimum SSF parameters for enzyme production have been determined. Various locally available agro-industrial residues have been screened individually or as mixtures for alkaline protease production in SSF. The combination of wheat bran (WB) with chopped date stones (CDS) (5:5) proved to be an efficient mixture for protease production as it gave the highest enzyme activity (90.50 U g(-1)) when compared to individual WB (74.50 U g(-1)) or CDS (69.50 U g(-1)) substrates. This mixed solid substrate was used for the production of protease from Streptomyces sp. CN902 under SSF. Maximal protease production (220.50 U g(-1)) was obtained with an initial moisture content of 60%, an inoculum level of 1 x 10(8) (spore g(-1) substrate) when incubated at 45 degrees C for 5 days. Supplementation of WB and CDS mixtures with yeast extract as a nitrogen source further increased protease production to 245.50 U g(-1) under SSF. Our data demonstrated the usefulness of solid-state fermentation in the production of alkaline protease using WB and CDS mixtures as substrate. Moreover, this approach offered significant benefits due to abundant agro-industrial substrate availability and cheaper cost.

No clear correlation between studied enteric viruses and coliphages concentration was proved. Coliphages abundance in collected samples should raise concerns about human enteric viruses transmission as these residues are reused in agricultural fields.

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