Mesenchymal Stem Cells (MSCs) under TNF-α stimulation (MSC-CM-T) can release numerous trophic and survival molecules that have a promising prospect in wound healing acceleration. However, the effective levels of MSC-CM-T in topical gel preparation to accelerate wound healing should be further explored. The aim of this study was to investigate the effects of MSC-CM-T in topical gel preparation in accelerating optimal wound healing through analyzing PDGF levels, wound closure rate percentages, and fibroblast density appearances. Twenty-four male Wistar rats were performed a full-thickness excision. The group studies were randomly assigned into four subgroups: control gel, control medium, and two treatment groups: MSC-CM-T topical gel at doses of 100 μL and 200 μL (T1 and T2, respectively). Wound closure rates were measured by standard caliper, platelet-derived growth factor (PDGF) levels were analyzed using ELISA on days 3 and 6, whereas the fibroblast density appearances were determined using hematoxylin-eosin staining. This study found a significant increase in PDGF levels in all treatment groups on days 3 and 6. These findings were in line with the increase of wound closure rates in all treatment groups on day 6, in which the high dose of MSC-CM-T was more effective in initiating the increase of wound closure rate. We also found the fibroblast density appearances on day 6 in the T2 group. We conclude that the topical gel of MSC-CM-T is more effective in accelerating wound closure healing through increasing PDGF levels and wound closure percentages and fibroblast density appearances in the skin defect animal models.

Food safety atau keamanan pangan merupakan kondisi dan upaya yang diperlukan untuk mencegah pangan dari kemungkinan kerusakan akibat cemaran biologis, kimia dan fisika. Kerusakan kimia pada makanan dapat terjadi dengan pemakaian berulang minyak jelantah yang menimbulkan efek negative bagi kesehatan dan jika dibuang dapat mencemari lingkungan. Kegiatan pengabdian masyarakat ini bertujuan membuat materi dan memberikan pelatihan untuk meningkatkan pengetahuan dan sikap pengolahan minyak jelantah menjadi sabun (sabun mije). Metode yang dilakukan pada kegiatan ini adalah penyusunan materi dan pelatihan pembuatan sabun mije. Materi panduan yang disusun berupa bahan presentasi dengan PPT, leaflet dan video demonstrasi. Sebelum dan setelah pelatihan diukur pengetahuan dan sikap peserta dengan kuesioner. Data hasil kuesioner akan dianalisis univariate untuk melihat karakteristik responden dan bivariate dengan uji t berpasanagn untuk menilai peningkatan pengetahuan dan uji Mc nemar untuk menilai perubahan sikap. Kegiatan pelatihan dilakukan di (RPTRA) Harapan Mulia, Kemayoran Jakarta Pusat dengan diikuti 20 peserta. Pengetahuan peserta sebelum dan sesudah pelatihan meningkat dari rata-rata 46,25 menjadi 72,65 (p=0,000). Perubahan sikap juga terjadi dimana sebelum pelatihan sikap positif sebanyak 45 % menjadi 85% setelah pelatihan (p=0,031). Peningkatan pengetahuan dan sikap yang significan ini diharapkan dapat meningkat peningkatan perilaku yang baik dalam pengolahan limbah minyak jelantah. Kata Kunci. Pengolahan limbah, pengetahuan, sikap, minyak jelantah, sabun mijeAbstractThe use of used cooking oil for cooking can cause negative effects for health. The used cooking oil when disposed can pollute the environment. This community service aims to create materials and provide training for the enhancement of the knowledge and attitude of the processing of used cooking oil to soap. The methods performed on this activity are training of soap making. This activity was done Kelurahan Harapan Mulia, Kemayoran, central Jakarta with the target of 20 housewives. The guide material is composed of presentation materials with PPT, leaflets and video demonstrations. Before and after training measured participants knowledge and attitudes with the questionnaire. The Data analyzed univariate to see the characteristics of respondents and bivariate with a postwar test to assess the increase in knowledge and test Mc Nemar to assess the change in attitudes. There were increasing knowledge from an average of 46.25 to 72.65 (P = 0,000). Attitude changes also occurred before the training of positive attitudes as much as 45% to 85% after training (P = 0,031). The increased knowledge and attitudes that are statistic significan are expected to increase the increase in good behaviour in the processing of household waste, especially processing the waste oil. Keywords : Sewage treatment, knowledge, attitude, cooking oil

Tuberculosis (TB) is a contagious disease caused by the group of Mycobacterium bacteria, Mycobacterium tuberculosis. There are six countries with the highest number of TB incidents in the world, including Indonesia with a prevalence of 1,020 per 100,000 inhabitants located second after India. Based on the results of monitoring and evaluation of Tuberculosis External Monitoring Mission Team in 2008, the success of treatment is still low. Many factors that influence the success of tuberculosis treatment one of them is the level of knowledge. This study aims to see and prove whether there is a relationship of knowledge level to the success of treatment in patients with tuberculosis and done cross-sectional. The result of the research shows that there are 45 respondents (80,4%) and the knowledge level of the respondent has good knowledge, 22 people (39,3%) from 56 samples. From the results of statistical tests using Chi-Square test obtained P value> 0.05 is 0.069.

Tuberculosis is a disease of global concern. By 2015 six countries contributing to 60% of the global total are India, Indonesia, China, Nigeria, Pakistan and South Africa. China, India and Indonesia alone accounted for 45% of cases in the world.The World Health Organization (WHO)has recommended the Directly Observed Treatment Shortcourse (DOTS) strategy for TB control by involving Drug Supervisors (PMO). It aims to achieve patient recovery, prevent transmission, and avoid drug resistant cases. This study aims to determine the relation between the roles of treatment observers (PMO) with the success of tuberculosis treatment at community health center of Johar Baru Central Jakarta in 2016.This research was conducted by Cross-Sectional non-experimental quantitative method. Population and sample are the patient of adult pulmonary and extrapulmonary tuberculosis at community health center of Johar Baru Central Jakarta in 2016. Samples are selected by using Simple Random Sampling. The data were collected by interview using questionnaire. Data analysis using SPSS with Chi-Square test.There were 45 respondents (80,4%) succeed in TB treatment and respondent with PMO roles category were 40 (71,4%). Result of statistical test using Chi-Square test obtained P value = 1,000 (> 0,05).There is no relation between the roles of treatment observers (PMO) with the success of tuberculosis treatment at community health center of Johar Baru Central Jakarta in 2016.

Background: Typhoid fever remains a health problem, particularly in Indonesia and other developing countries. There are few selection of antibiotics that used for the treatment of typhoid fever.Objective: The purpose of this research was to determine the antibiotic used in the treatment of typhoid fever without complications in children at Putera Bahagia Hospital Cirebon and to see the difference effectiveness.Methods: The research was conducted in retrospective, that is taking medical records of pediatric patients aged 1-15 years with a diagnosis of typhoid fever at Putera Bahagia Hospital, Cirebon, periode of 2011-2014. Statistical analysis was performed using Kruskal-Wallis test, with SPSS 23 program. Results: Found 117 cases that met the inclusion criteria and antibiotics used are chloramphenicol (23.08%), cefixime (11.97%), cefotaxime (11.11%), ceftriaxone (49.57%), and azitromisin (4.27%). The fastest fever’s free-time is ceftriaxone, 42.46 hours (average time) and the shortest treatment duration is azithromycin, which is 4.8 days.Conclusion: In this research, there was no significant difference between fever’s free-time and treatment duration of chloramphenicol, cefixime, cefotaxime, ceftriaxone, and azithromycin.