Microbial and opportunistic pathogens cause serious problem to human host by releasing different compounds which are involved in colonization, invasion or in alteration of immune process. In this study we propose ourselves to analyze the virulence profile of microorganisms involved in urinary tract infections and bacterial vaginosis in order to compare the virulence mechanisms developed by these microorganisms. We assessed the virulence profile (motility, adhesion to HeLa cell line, biofilm formation, production of enzymes, antibiotic susceptibility) using phenotypic methods. The microbial strains isolated from urinary tract infections present a high ability to release amylases, caseinase, siderophore-like, half of them have present high motility and also were highly resistant to antibiotics, while the microbial strains isolated from bacterial vaginosis present a high ability to bind human epithelial cells and to release hemolyzin and DN-ase.

The high frequency of occurrence of candidiasis as well as high mortality of patients with immunosuppression cause a tendency toward better understanding of Candida albicans species virulence factors and developing sensitive and specific diagnostic methods, and appropriate strategies of candidiasis treatment. In recent decades the incidence of fungal infections has alarming increases because of advanced medical treatments. In this study was analyzed possible ultrastructural changes of the species C. albicans cells following treatment with sodium diclofenac at various concentrations. Following treatment of C. albicans cells with sodium diclofenac 1 mM and 2 mM changes in the plasmalemma can be noticed, changes in the density of cell wall, disruption and necrotic appearance of the cytoplasm.