The bioavailability of pollen bioactive compounds for humans is limited. In this study, our aim was to enhance the health-related benefits of pollen by fermentation with a Kombucha/SCOBY (symbiotic culture of bacteria and yeasts) consortium. We performed the fermentation of pollen suspended from the beginning with SCOBY on sweetened green tea or on Kombucha vinegar, by adding pollen after 20 days of Kombucha fermentation. We analyzed: formation of bioactive compounds (anti-oxidant polyphenols, soluble silicon, hydroxy-acids, short chain fatty acids—SCFA); parameters related to Kombucha fermentation (dynamics of lactic acid bacteria—LAB, formation of organic acids, soluble sugar evolution on Kombucha vinegar); the influence of Kombucha fermentation on pollen morphology and ultrastructure; in vitro cytotoxic and antitumoral effects of the Kombucha fermented pollen. The pollen addition increases LAB proportion in the total number of SCOBY microbial strains. SEM images highlight the adhesion of the SCOBY bacteria to pollen. Ultrastructural analysis reveals the release of the pollen content. The content of bioactive compounds (polyphenols, soluble silicon species and SCFA) is higher in the fermented pollen and the product shows a moderate antitumoral effect on Caco-2 cells. The health benefits of pollen are enhanced by fermentation with a Kombucha consortium.

The aim of this study was to improve the bovine colostrum biological function through fermentation with kefir grains enhanced with selected yeasts, for developing new nutraceutical and cosmeceutical products. It was found that fermentations with coculture of 2.5 g% artisanal kefir grains and selected yeast strains (10 6 CFU/100 mL) increased the functional quality of the fermented products compared to the product obtained only with kefir grains. Fresh fermented products obtained with a consortium based on kefir grains and Candida lipolytica MIUG D67 demonstrated an increased antioxidant activity of 2.69 mM Trolox Equivalent/g, after 48 h of fermentation. Instead, peptide fractions with MW<10 kDa isolated by membrane filtration from lyophilized fermented products, based on colostrum fermentation with kefir grains enhanced with Candida lipolytica MIUG D99 starter, presented markedly increase in vitro of ABTS radical scavenging activity, similar to a concentration of 2 nM captopril. These results indicated their possible application in enhance of the quality of the fermented products in order to increase the postbiotic composition with functional impact in vivo.

No abstract

Many western societies are confronted with issues in planning and adapting their health policies due to an ageing population living alone. The “NOt Alone at Home—NOAH” project aimed to involve older people in the Agile co-creation of services for a collaborative monitoring and awareness notification for remote caregivers. Our research aim was to create a scalable and modern information system that permitted a non-invasive monitorization of the users for keeping their caregivers up to date. This was done via a cloud IoT (Internet of Things), which collects and processes data from its domotic sensors. The notifications generated by the system, via the three applications we developed (NOAH/NOAH Care/Admin Centre), offer caregivers an easy way of detecting changes in the day-to-day behaviour and activities of their patients, giving them time to intervene in case of abnormal activity. Such an approach would lead to a longer and more independent life for the older people. We evaluated our system by conducting a year-long pilot-study, offering caregivers constant information from the end-users while still living independently. For creating our pilot groups, we used the ABAS (Adaptive Behaviour Assessment System) II, which we then matched with the pre-profiled Behavioral Analysis Models of older people familiar with modern communication devices. Our results showed a low association between daily skills and the sensors we used, in contrast with the results from previous studies done in this field. Another result was efficiently capturing the behaviour changes that took place due to the COVID-19 Lockdown measures.

As technology has evolved, the field of liquid chromatography has developed, bringing new, alternative and high level methods of separation, isolation and purification of the natural products present in the natural plant extracts, replacing the traditional chromatographic methods. In this direction, until now, it has been observed that the Centrifugal Partition Chromatography (CPC) presents a high precision in isolating the individual compounds. Thus, we chose to study this topic in the present review. Currently, there is a growing interest in the use of CPC in the sustainable isolation and purification of cannabidiol (CBD) from Cannabis plants due to the extensive medical benefits of cannabis compounds (i.e. epilepsy, multiple sclerosis, etc.