Aim: The objective of this study is to determine the applications of nurses for port catheter care. Material and Method: The research was conducted as a descriptive study. The study sample consisted of 196 nurses who worked in an Education and Research Hospital and agreed to participate in the study. In this study, data were collected using a data collection form created by researchers. Statistical data were expressed as mean±standard deviation (X±SS) and percentage (%). Results: According to the results of this study, 90.8% of nurses were women, 65.8% were married, 69.4% had a bachelor’s degree, and the average age was 38.08±8.76. 77.04% of nurses (n=151) are concerned about using a port catheter as a venous access point in a patient with a port catheter. 15.81% of the nurses (n=31) stated that they use port catheter right after controlling its location through radiography, 17.34% of the nurses (n=34) stated that pulling back blood on the syringe to confirm the location of the port catheter by observing the fluid flow, 20.4% of the nurses (n=40) state that they used a specific catheter needle to intervene the port catheter, 39.28% of the nurses (n=77) stated that they flushed the port catheter with saline and heparin saline to avoid clogging of the catheter. They flushed the catheter port once in 1-2 months with heparin saline when the catheter port cannot be used for a long time. 21.93% (n=43) of nurses stated that they dressed the area with antiseptic solution before application to prevent port catheter infection, and 17.34% (n=34) of them stated that they checked the location of the catheter by withdrawing blood before application to prevent extravasation. Conclusion: The applications used by the nurses for port catheter care are compatible with the literature; however, these are limited.