Mango fruits from different varieties vary in aroma and taste. In this study, methanol extracts of fruit pulps obtained from seven Indian varieties of Mangifera indica (Amrapali, Fazli, Golapkhas, Gopalbhog, Himsagar, Langra, and Mohanbhog) were profiled using a metabolomics approach. Chemometric methods were used to understand the contribution of metabolites to varietal differences. The extracts were also assayed for pancreatic lipase inhibitory activities in vitro. All the pulp extracts were analyzed by GC-MS and HPLC to detect their metabolites. In sum, 63 metabolites were identified. The varieties were distinctly different chemically in terms of the identified metabolites, as revealed by principal component analysis (PCA) and partial least squaresdiscriminant analysis (PLS-DA). From the biplot and VIP scores, it was observed that in addition to some organic acids, amino acids, sugars, and phenolic constituents (e.g., benzene-1,2,4-triol, mangiferin, and pyrogallol) contributed to this variation. All the varieties also inhibited pancreatic lipase in a dose-dependent manner. Gallic acid (IC 50 value 0.47 nM) and 4-hydroxy benzoic acid (IC 50 value 1.15 nM) showed high anti-lipase activity. Three amino acids (L-proline (IC 50 value 0.01 µM), L-alanine (IC 50 value 0.14 µM) and aspartic acid (IC 50 value 0.02 µM)), and the inorganic acid phosphoric acid (0.02 µM) also showed activity against pancreatic lipase. ARTICLE HISTORY

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Background: Methanolic extracts of the stems of six species of Berberis e.g., Berberis umbellata, B. vulgaris (Syn: B. nepalensis), B. insignis, B. asiatica, and B. aristata were studied for their antioxidant properties and acetylcholinesterase inhibitory properties. Materials and Methods : Antioxidant property was studied in different systems of assay e.g., DPPH radical scavenging assay, superoxide radical scavenging assay, nitric oxide scavenging assay, assay to chelate metal. Acetylcholinesterase (AChE) inhibitory property was measured using AChE from electric eel. Results: It was observed that all the extracts scavenged DPPH, superoxide and nitric oxide radical and chelated metal ion in a dose dependent manner. The antioxidant properties were correlated with total phenol content, total flavonoid content, berberine and palmatine content. The AChE inhibitory activities of the extracts were also correlated well with berberine, palmatine, total phenol and total flavonoid content. A few phenolic compounds were detected by GC-MS. Highest activity in all respect was observed in B. aristata. Conclusion: Methanolic extracts of the stems of B. aristata exhibited the highest antioxidant activity.