Rhizosphere fungi are strongly associated with plant growth and health by providing nutrients and antagonizing pathogens. Commercially, fungus has multipurpose applications in several sectors including beverages, food items and in medicines. Current study aimed to reveal the core fungal community structure of the two leading cereal crops that are Zea mays L. and Triticum aestivum L. The rhizosphere fungal community was explored via morphology, biochemistry and internal transcribe spacer (ITS) metagenomics. On the basis of morphology, the retrieved fungal strains were imprecisely classified into Ascomycota and Zygomycota. The species including Yeast, Botyritis californica, Rhizopus stolonifer, Alternaria tenuissima, Aspergillus terreus, Aspergillus flavus, Aspergillus nidulans, Aspergillus niger and Microsporum canis were identified on the basis of macroscopy and microscope. Moreover, the biochemical characterization depicted the role of fungi in promotion of plant growth. Majority of the isolates depicted catalase activity, indole production, phosphate solubilization, ammonia production, nitrogenase activity and urease activity. Metagenomics using amplicon sequencing of ITS region revealed the presence of 805 Operational Taxonomic Units (OTUs) with 647 OTUs in Zea mays and 620 OTUs in Triticum aestivum. The fungal phyla found in the rhizosphere of Zea mays L. and Triticum aestivum L. were Ascomycota, Basidiomycota, Zygomycota, Chytridiomycota, Incertae sedis fungi. Ascomycota accounted for 93% and 95% of classified fungi in rhizosphere of Zea mays L. and Triticum aestivum L. respectively. The dominant species found in the rhizosphere soil of Zea mays were Gibberella intricans, Curvularia lunata, Lepidosphaeria nicotiae, Edenia gomezpompae and Myrothecium verrucaria.
Excessive use of chemical fertilizers is common for higher production of Groundnut (Arachis hypogea L.). Chemical fertilizers are costly andhave adverse effect on soil environment. Keeping in view avoidingthe side effects of chemical fertilizers, an experiment was carried out for evaluating the different organic manures to select potential organic manure for better groundnut production during 2018-19 at Groundnut Research Station Attock. The experiment was laid out in split plot design with three replications, fivetreatments consisted of different organic and inorganic fertilizers i.e. T1 (FYM @7.5t/ha), T2 (PM @05t/ha), T3 (NPK @30:80:30 kg/ha), T4 (Vermicompost@ 2.5t/ha) and T5 control (zero fertilizer). Nutrients use efficiency, quality and yield components were recorded. The study revealed that organic manure can enhance the production and quality of Ground nut for replacing the chemical fertilizer. Poultry manure @ 05t/ha significantly produced higher yield (4203 kg/ha), shelling% (71%), 100 seed weight (72.66 g), plant height (31.66 cm), pods/plant(58.00), oil content (42.86%), protein(28.60%) during both the years over rest of treatments. Similarly FYM and vermicompost had better results than the NPK. Therefore it is recommended that poultry manure @05t/ha can improve groundnut yield under pothohar conditions of Pakistan.
The present study was conducted at four different locations for the evaluation of mung bean (vigna radia L.) genotypes. It was shown that all of the genotypes performed better in producing yield at Sarghoda region of Pakistan. The mung bean genotype MPP-15039 performed highest in Sarghoda region and produce high yield in all other three location of the Pakistan in comparison with other genotypes. In Faisalabad region all the genotypes performed on second number in producing mung bean yield as compared to other region. The production of the genotypes was on third position at Sialakot followed by Faroqabad. It was also depicted from the study that the genotypes producing more pods also produced more yield. The Genotype MPP-15039 was producing more pods in Sargodha region and it produced more yield. The genotypes producing less number of the pods also produce low yield as in MPP-15002 as shown in graph 3.
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