Background: Polymethyl methacrylate (PMMA) bone cement is the clinical gold standard biomaterial for local antibiotic therapy in osteomyelitis. However, it releases 50% of the antibiotic within first three days. It generates excessive heat during polymerization and is non-biodegradable. It must be removed by another operation. The best-known alternative for PMMA is hydroxyapatite. Objective: The present work is focused to synthesize the biodegradable hydroxyapatite in nano form for slow and sustained release of antibiotics and to study the release kinetics of antibiotics. Method: Nano-hydroxyapatite was synthesized by co-precipitation method and characterized by particle size analyser, transmission emission microscopy, fourier transform infrared spectroscopy and energy dispersive X-Ray analysis. Antibiotic loaded nano-hydroxyapatite was prepared as 7 mm beads. The efficiency of drug loaded nano- hydroxyapatite beads against osteomyelitic isolates were evaluated by well diffusion assay. Zero order, first order, second order, Higuchi model Korsmeyer-Peppas and Gompertz model were fit into the release kinetics of antibiotics from hydroxyapatite. Results: Average size of nano-hydroxyapatite was 5 nm. The bactericidal activity exhibited by antibiotic loaded micro sized hydroxyapatite was therapeutic until 10 days only whereas antibiotic loaded nano-hydroxyapatite was therapeutic until 8 weeks. This confirms the burst release of antibiotics from micro sized hydroxyapatite beads. In contrast, the release was slow and sustained upto 8 weeks from nano-hydroxyapatite. Koresmeyer-Peppas model fits into the release kinetics of antibiotics from nano-hydroxyapatite. Conclusion: Nano-hydroxyapatite with Ca/P ratio of 1.78 is suitable for the slow and sustained delivery of antibiotics for 8 weeks.