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Aim: This study aimed to determine the effect of the menstrual attitudes of female nursing students on their genital hygiene behaviors. Methods: The cross-sectional study was conducted with 341 female student in the 2019-2020 academic year. The data were collected by "Personal Information Form'', "Genital Hygiene Behavior Scale'' and "Menstruation Attitude Scale''. Descriptive statistics, OneWayAnova test, correlation and regression analysis methods were used in analyzing the data. Results: In this study 78,9 of the respondents were 20 years and above, 39,9% of them were in the first class. Most of them (64,5%) were lived in the dormitory. It was determined that the mean total score of the students on the Menstrual Attitude Scale was 93,31±10,27 and genital hygiene behaviors scale was 95,50±9,42. It was found that there is a positive relationship between the perception of menstruation as a natural phenomenon and genital hygiene behaviors and menstrual hygiene. As a result of the regression analysis, it was found that the number of pads used was effective on general hygiene behavior and awareness of abnormal findings, while the length of menstruation and the number of pads were effective on menstrual hygiene. Conclusion: It was found that students' perception of menstruation as a natural phenomen on positively affected their menstrual hygiene behavior. Accordingly, it is thought that positive attitudes about menstruation from the first experience will positively affect the general health status of women in all life periods.