The administration of renin to the rabbit or the rat causes marked changes in the composition of the urine (1-3). The adult female rat normally excretes 0.1 to 0.2 mg. of protein in its urine per hour, while the adult male rat excretes from three to five times this amount (4). However, in the hour following the intraperitoneal injection of 4 Goldblatt dog units of hog renin the excretion of protein increased to an average value of 31.7 rag. (5). Gilson (6) fraetionated the urinary protein of normal rats with ammonium sulfate, and found approximately equal quantities of albumin and globulin. Wicks (7) found traces of albumin and globulin in normal mouse urine, but concluded that the greater portion of the urinary protein consisted of nucleoprotein. Parfentjiv and Perlzweig (8) reported that the protein present in the urine of normal male mice was a chondromucoid substance. The nature of the protein excreted in the urine following renin administration is not known. The present report describes an electrophoretic study of normal urinary proteins in the rat, and of the proreins appearing in the urine following the administration of renin. MethodsAdult male and female albino rats of the Slonaker-Addis strain weighing from 150 to 200 gm. were used in these experiments. These animals were maintained on a diet containing 17 per cent protein and adequate quantities of all of the known essential food factors. Preparation of Normal Rat UrinaryProteins.--Groups of fifteen animals of the same sex were taken from the colony at 4:00 p.m. and placed in individual metabolism cages. The voided urine was collected during the ensuing 17 hours. The technique used for the collection of uncontaminated urine has been described elsewhere (2).

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