This study aimed to determine total phenolics, assess toxicity and antimicrobial activity of essential oil (EO) of Alpinia zerumbet leaves. The EO was extracted by hydrodistillation and the physicochemical parameters were determined according to the Brazilian Pharmacopoeia. The toxicity assay followed the lethality bioassay against the non-target organism Artemia salina Leach. The antimicrobial activity followed the methodology described by the Clinical and Laboratory Standards Institute using the Method of Disc Diffusion and Broth Dilution to determine the Minimum Inhibitory Concentration (MIC) for the action of EE against Escherichia coli, Staphylococcus aureus, Aspergillus sp. and Penicilium sp. OE yield was 0.71%. Lethal Concentration 50% (LC50) for the action of EO against Artemia salina L. 280.40 mg/L, being considered nontoxic. Through the antimicrobial assays, the OE presented bactericidal activity against the bacteria E. coli and S. aureus,and fungicide activity against the fungi Aspergillus sp. and Penicillium sp. It was concluded that the EO has a good antimicrobial potential, and its application is appreciated by its classification as nontoxic OE..
Introduction: This study evaluated the chemical characterization, larvicidal activity and molluscicidal activity in front of the snail transmitting schistosomes (Biomphalaria glabrata) of the essential oils of Alpinia zerumbet and Cymbopogon citratus (DC.) Stapf. The essential oils (EOs) were extracted by hydrodistillation, with chemical characterization through Gas Chromatography coupled to mass spectrometry (GC-MS). The physical-chemical parameters were determined according to the Brazilian Pharmacopoeia. The toxicity test followed the bioassay with Artemia salina Leach, the EOs approved in this assay followed to evaluate their biological properties. Methodology: for molluscicidal activity, the methodology recommended by the WHO was performed, and the LC50 of the EOs was performed for their action in the face of the snail obtained by the Reed&Muench method. Both EOs showed low toxicity, and thus were evaluated for the biological properties larvicidal and molluscicidal .Alpinia zerumbet EO showed molluscicidal activity with LC50 of 40.63 mg·L-1 and Cymbopogon citratus EO 33.94 mg·L-1. Results: Both EOs showed larvicidal and molluscicidal potentials against the organisms tested, showing satisfactory results for their action. The results indicate that the evaluated EOs are composed of substances that promote and encourage their application due to their potential for molluscicidal and larvicidal biological activity.
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