IntroductionWith revolutions in Information Technology, information and misinformation are easier to be found online. YouTube is the largest and most commonly searched video content website in the world. It is assumed that, due to the coronavirus pandemic, most patients try to know about diseases through the internet and reduce the number of hospital exposures unless otherwise. In order to assess the understandability and actionability of such YouTube videos available freely online about the disease, Hemolytic disease of the newborn (HDN), this study was planned. MethodsThis is a cross-sectional study conducted with the first 160 videos available on May 14, 2021, with the search keyword "HDN" with a relevance filter and a duration of 4 to 20 minutes. The videos were further screened regarding the information content and language. These videos were assessed by three independent assessors using the patient educational materials assessment tool for audio-visual content.