No abstract

Dermal fibroblasts (dFBs) are an essential component of skin; they not only produce and organize the extracellular matrix of the dermis, but also are essential for wound healing, hair growth, fibrosis and defense against infection. Fibroblast heterogeneity has long been recognized in mouse and human skin, but the cellular hierarchy and mechanisms governing fibroblast heterogeneity are incompletely understood. Here, we used single-cell RNAsequencing to study how cellular heterogeneity of murine skin is turned at the transcriptional level during post-natal periods using single cells isolated from the skin of new born (P1), young (3 weeks) and adult (2 month) mice. Unbiased clustering of >10,000 single-cell transcriptomes revealed 29 distinct population of the skin. Within these clusters, Pdgfra clearly marked 13 dFB clusters, which were then re-clustered into 23 dFB clusters. Pseudotime analyses of these dFB clusters identified a Pdgfra + CD24 hi Thy1 lo Sca1 lo progenitor population that was highly abundant in neonatal skin early in life but declined in adulthood. Pseudotime analyses revealed that this progenitor population gave rise to several FB subtypes, including dermal papillary FBs, reticular FBs that produce high levels of type1 collagen, adipocytes that produce antimicrobial peptide Camp as well as the interstitial reticular FB that are enriched with inflammatory gene signature during post-natal development. The ability of this progenitor population to commit to collagen 1 producing reticular dFB and to differentiate into adipocytes was confirmed by primary dFB culture in vitro. Together, our study allows the reconstruction of gene expression programs during fibroblast development and provides insights into how fibroblasts develop heterogeneity from progenitors during adulthood.

AbstrakSebanyak 30-50% pasangan khawatir hubungan seksual akan membahayakan janinnya. Tujuan penelitian ini untuk mengetahui pengaruh pendidikan seksual terhadap perilaku pemenuhan kebutuhan seksual pasangan masa kehamilan di Puskesmas Kasihan II Bantul. Penelitian menggunakan rancangan quasi-eksperimental metode non-equivalent control group design. Sampel terdiri 26 subyek kelompok eksperimen dan 26 subyek kelompok kontrol, dengan metode purposive sampling. Instrumen berupa kuesioner yang telah diuji validitas korelasi product moment dan uji reliabilitas alpha cronbach. Analisis data menggunakan paired samples t-test dan independent samples t-test. Hasil uji normalitas data menggunakan kolmogorov-sminorv p>α=0,05, sehingga data terdistribusi normal. Hasil analisis paired t-test kelompok eksperimen adalah p<0,05, hasil kelompok kontrol p>0,05 dan hasil independent samples t-test kelompok eksperimen dan kelompok kontrol yaitu p<0,05. Disimpulkan terdapat pengaruh signifikan pemberian pendidikan seksual terhadap perilaku pemenuhan kebutuhan seksual masa kehamilan di Puskesmas Kasihan II Bantul. Sexual Education and Sexual Behavior in Couples During Pregnancy

Background Spinal muscular atrophy is a genetic disorder characterized by degeneration of lower motor neurons, leading to progressive muscular atrophy and even paralysis. Spinal muscular atrophy usually associated with a defect of the survival motor neuron 1 (SMN-1) gene. Classification of spinal muscular atrophy is based on the age of onset and maximum motor function milestone achieved. Although spinal muscular atrophy can be screened for in newborns, and even confirmed earlier genetically, this remains difficult in Third World countries such as Indonesia. Case presentation A 28-year-old Asian woman in the first trimester of her second pregnancy, was referred to the neurology department from the obstetric department. Her milestone history showed she was developmentally delayed and the ability to walk independently was reached at 26 months old. At 8 years old, she started to stumble and lose balance while walking. At this age, spinal muscular atrophy was suspected because of her clinical presentations, without any molecular genetic testing. She was married at the age of 25 years and was soon pregnant with her first child. At the gestational age of 32 weeks, her first pregnancy was ended by an emergency caesarean section because of premature rupture of the membranes. In this second pregnancy, she was referred early to the general hospital from the district hospital to receive multidisciplinary care. She and her first daughter underwent genetic testing for spinal muscular atrophy, which has been readily available in our institution since 2018, to confirm the diagnosis and prepare for genetic counseling. Conclusions Managing pregnancy in a patient with spinal muscular atrophy should be performed collaboratively. In this case, genetic testing of spinal muscular atrophy and the collaborative management of this patient allowed the clinical decision making and genetic counseling throughout her pregnancy and delivery.