There are many activities and uses in the coastal environment, which has historically attracted the humans. This attraction has led to many anthropic actions that have generated imbalances, more important as the human pressure increases. This research focuses on the effects of these pressures along of 11km of the coastline of Guardamar del Segura, a high-value environmental area where is the Segura River mouth and one of the last dune systems of the southeast of Spain. The historic evolution of the shoreline position has been analysed using 60years of aerial images from 1950s to 2014, the seabed depth changes, the maritime climate, the distribution of the sediment grain size and the anthropic actions such as urban development or the channelling of the river. All data were integrated and processed using a Geographic Information System (GIS). The results show that the lack of sediment supply by Segura River and the cut-off in the longshore transport due to the breakwaters and others anthropic actions has led into an increase in the beaches erosion rates, with a loss of >3.2millionm of sand in the last 58years (≈55,200m/year). The conclusions of this research could be useful to the coastal managers at the moment of making the decisions of action and/or conservation on a coastal system to achieve positive results in the medium and long term.

No abstract

Adrenal chromaffin cells contain at least two subtypes of nicotinic acetylcholine receptors (nAChRs). These studies were designed to identify and characterize the subtype of nAChR mediating adrenal catecholamine release using the monoclonal antibody mAb35, which recognizes the a-subunit of muscle nAChRs and crossreacts with some neuronal nAChRs. Immunocytochemical studies demonstrated that mAb35 interacts with specific sites on cultured chromaffin cells. Pretreatment with mAb35 reduced nAChR-stimulated catecholamine release (1050 of '-10 nM). mAb35 had no effects on release stimulated through non-nAChR mechanisms. Unlike agonist-induced nAChR desensitization, the mAb35-induced reduction in nAChR-mediated secretion developed slowly. Although not immediately reversible, nAChR-stimulated release recovered after mAb35 removal. However, unlike recovery from agonist pretreatment, recovery from mAb35 pretreatment was relatively slow and was partially blocked by vinblastine. Hybridization of adrenal chromafin RNA with a rat a3 cDNA revealed two strong bands and two fainter bands: two higher-molecular-weight bands, 6.9 and 8.5 kb; a strong band of 3.2 kb; and a lower amount of a 2.3-kb RNA. With recovery of nAChR function after agonist or mAb35 treatment, no significant effects on a3 subunit mRNA levels were seen. In summary, these studies demonstrate the presence of mAb35-nAChRs on adrenal chromaffin cells and provide evidence that these receptors represent the major population that regulates secretory events in adrenal chromaffin cells. Key Words: Adrenal chromaffin cells-mAb35-Catecholamine secretion -Nicotinic acetylcholine receptors-a3 mRNA.

is widely distributed in the tropics and subtropics. The aim of this study was to determine the prevalence of and other intestinal parasites and identify the risk factors for infection with in a rural area of Angola. A cross-sectional study was conducted in school-age children (SAC) in Cubal, Angola. A questionnaire collecting clinical and epidemiological variables was used, and two stool samples were collected. A concentration technique (Ritchie) and a technique for detection of larvae migration (Baermann) were performed. Of 230 SAC, 56.1% were female and the mean age was 9.3 years (SD 2.45). Severe malnutrition, according to body mass index (BMI)-for-age, was observed in 20.4% of the SAC, and anemia was found in 59.6%. was observed in 28 of the 230 (12.8%) SAC. Eggs of other helminths were observed in 51 (22.2%) students: spp. in 27 students (11.7%), hookworm in 14 (6.1%), four (1.7%), in four (1.7%), in three (1.3%), spp. in two (0.9%), and one (0.4%). Protozoa were observed in 17 (7.4%) students. Detection of was higher using the Baermann technique versus using formol-ether (11.3 vs. 3%). Overall prevalence of in the school population of 16 studied schools in the municipal area of Cubal was greater than 10%. This fact must be considered when designing deworming mass campaigns. The use of specific tests in larvae detection is needed to avoid overlooking this parasite.