Grape pomace (GP) is a polyphenolic-rich byproduct of wine production. As most polyphenolics are either bound to cellular matrices or present as free polymeric forms, treatment with hydrolytic enzymes may act to increase GP functionalities. The aim of this study was to examine the impact of tannase alone (T), pectinase plus cellulase (PC) or a mixture of them (TPC) on the hydrolysis of polyphenolics in red GP (RGP), white GP (WGP), and mixed GP (MGP) from Brazilian wine production, as well as antioxidant activity of the products. T was the most potent in increasing total polyphenols in GP by liberating gallic acid, caffeic acid, quercetin, and trans-resveratrol. PC increased the catechin content in RGP and TPC increased the procyanidin B2 in WGP. T treatment of GP was most effective in increasing antioxidant activity. In conclusion, the enzymatic treatment, particularly with T, increases the polyphenolic content and antioxidant activity of GP.
Dysfunction of the intestinal barrier plays a key role in the pathogenesis of inflammatory bowel disease (IBD) and multiple organ failure. The effect of Alaska pollock skin-derived collagen and its 3 tryptic hydrolytic fractions, HCP (6 kDa retentate), MCP (3 kDa retentate) and LCP (3 kDa permeate) on TNF-α induced barrier dysfunction was investigated in Caco-2 cell monolayers. TNF-α induced barrier dysfunction was significantly attenuated by the collagen and its peptide fractions, especially LCP, compared to TNF-α treated controls (P < 0.05). Compared to a negative control, 24 h pre-incubation with 2 mg mL LCP significantly alleviated the TNF-α induced breakdown of the tight junction protein ZO-1 and occludin and inhibited MLC phosphorylation and MLCK expression. The activation of NFκB and Elk-1 was suppressed by LCP. Thus, collagen peptides may attenuate TNF-α induced barrier dysfunction of Caco-2 cells by inhibiting the NFκB and ERK1/2-mediated MLCK pathway with associated decreases in ZO-1 and occludin protein expression.
Grape pomace (GP) is rich in polymeric polyphenolics and glycosides which have lower bioefficacy than monomeric and aglycone counterparts. The aim of this study was to determine whether tannin acyl hydrolase [tannase (TNS)] can improve the antioxidant and anti-inflammatory actions of GP in Caco-2 cells treated with IL-1β. TNS increased quercetin content by 45% and decreased quercetin-3-O-rutinoside by 71% as compared to untreated GP. Further, TNS increased total phenols and DPPH, ORAC, and FRAP values by 39, 57, 215, and 12%, respectively. However, GP and GPTNS at 100 and 200 µg/mL (dry extract wt/v) displayed comparable efficacy in the reduction of ROS in Caco-2 cells. After 24 h pre-treatment, GPTNS (200 µg/mL) decreased IL-1β-induced PGE 2 and IL-8 secretion by 107 and 83% respectively, and down-regulated NF-κB activation by 63%. Thus, TNS appears to enhance antioxidant and anti-inflammatory activities of GP polyphenolics and suggests their use as a value-added agroindustrial residue.
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