Abstract. With the increased use of horses in sports of various modalities, sports medicine has been increasingly studied so as to have a better understanding of what happens to these animals during the proposed activity. Fitness and training are essential for proper performance and performance in equestrian events, being an important role in the routine of these animals. The aim of the present study was to evaluate changes in serum biochemistry (creatine kinase, glucose and lactate) in seven Mangalarga Marchador (MM) horses submitted to gait training. Three collections were performed within 60 days, divided into D0, D30 and D60 with the animals at rest and after exercise. The results were: regarding pre-training, mean CK values were obtained in 152.2 ± 50.9 IU/L, 277.2 ± 194.4 IU/L and 301.9 ± 125, glucose in 92. 3 ± 11.4 mg/dL, 97.0 ± 10.3 mg/dL and 87.6 ± 10.3 mg/dL, and lactate at 2.4 ± 0.5 mmol/L, 2.0 ± 0.4 mmol/L and 2.2 ± 0.6 mmol/L, respectively at D0, D30 and D60. Post-training mean CK values were 187.9 ± 25.5 IU/L, 358.6 ± 244.1 IU/L and 307.6 ± 116 IU/L, glucose 97.3 ± 11, 4 mg/dL, 119 ± 16.8 mg/dL and 108.6 ± 28 mg/dL and lactate at 3.3 ± 0.9 mmol/L, 3.6 ± 1.2 mmol/L and 4 ± 1 mmol/L at D0, D30 and D60 respectively. No significant increase in CK was observed under the type of exercise imposed, increasing the powders by 14.4% in the average of all collections. Of the lactated results, we had a significant increase in the pre-training average compared to the post in 39.4%. Already glucose was not significant having increased after 14.8% in the average days of the present study. We can observe that the change in glycemia was relatively different from lactate levels demonstrating the use of the aerobic route.

The rural extension is a technical activity that aims at an educational and instructive character in assisted properties, especially for small and medium rural dairy farmers. In this way, among other factors, it aims to maintain the welfare and health of the animals involved and also to produce subsistence with the activity. Given the importance of dairy farming in Brazil and the various difficulties encountered in this sector, this work aims to report the performance and importance of the "Rural Extension-Dairy Cattle" Project of the Professional Improvement Program in Veterinary Medicine at the University of Franca (UNIFRAN). This extension project, created in 2012, in addition to improving the knowledge of recently graduated academics, provides veterinary technical assistance to small rural dairy farmers in the city of Franca (SP) and region, especially in relation to the processes that involve the handling, welfare, nutrition, production, reproduction and health of the animals. Each selected student develops these activities for two consecutive years, under the constant supervision and guidance of the institution's faculty, in addition to the assistance of interns. Thus, using the rural extension methodology recommended by the "Rural Extension-Dairy Cattle" Project, It is admitted that it provides the intensive improvement of the veterinarian for being directly inserted in the current job market, in addition to offering instructions to rural producers followed by animal care, which favors the productivity and quality of the product obtained.