The entomopathogen Bacillus thuringiensis (Bt) is widely used as one of the ingredients in pest control formulations, but researches conducted on its effect on non-target organisms are still in the nascent stage. This investigation aimed to uncover if Bt treated with Tenebrio molitor (Coleoptera: Tenebrionidae) larvae and pupae could affect the biological variables of Podisus nigrispinus (Hemiptera: Pentatomidae) and Palmistichus elaeisis (Hymenoptera: Eulophidae), all of which established natural enemies of leaf defoliator caterpillars in the eucalyptus culture. Larvae of T. molitor were fed on wheat bran containing different concentrations of B. thuringiensis (0.00; 0.25; 0.50; 1.00; 2.00 and 4.00 g Agree/kg bran). When the larvae attained size of about 2 cm, they were used as prey for P. nigrispinus (Bioassay I), and their pupae used as hosts for P. elaeisis (Bioassay II). Only the biological variables oviposition period and egg numbers by posture of the predator P. nigrispinus were altered. The biological variables of P. elaeisis were not altered, since it was possible to use these control methods within the integrated pest management.