YbeX ofEscherichia coli, a member of CorC protein family, is a putative Co2+/ Mg2+efflux factor. Here, we describe severalΔybeXphenotypes and report an involvement of YbeX in ribosomal metabolism.E. colilackingybeXhas a longer lag phase on outgrowth from the stationary phase. This phenotype is heterogeneous at the individual cell level and can be rescued by supplementing the growth media with magnesium.ΔybeXstrain is sensitive to elevated growth temperatures and to several ribosome-targeting antibiotics, which have a common ability to induce the cold shock response inE. coli.ΔybeXcells accumulate distinct 16S rRNA degradation intermediates present in both 30S particles and 70S ribosomes. We propose that a function of YbeX is maintaining the magnesium homeostasis in the cell, which is needed for proper ribosomal assembly.
Silicone is often used in environments where water repellency is an advantage. Contact with water promotes the adhesion of microorganisms and biofilm formation. Depending on the application, this may increase the possibility of food poisoning and infections, the material's degrading appearance, and the likelihood of manufacturing defects. The prevention of microbial adhesion and biofilm formation is also essential for silicone-based elastomeric foams, which are used in direct contact with human bodies but are often difficult to clean. In this study, the microbial attachment in and the retention from the pores of silicone foams of different compositions is described and compared to those of commonly used polyurethane foams. The growth of the gram-negative Escherichia coli in the pores and their leaching during wash cycles is characterised by bacterial growth/inhibition, adhesion assay, and SEM imaging. The structural and surface properties of the materials are compared. Despite using common antibacterial additives, we have found that non-soluble particles stay isolated in the silicone elastomer layer and, therefore, do not possess antibacterial activity. Water-soluble tannic acid dissolves into the medium and seems to aid in inhibiting planktonic bacterial growth, indicating that tannic acid is available on the surface of the SIFs to some extent.
Günümüzde üç fazlı asenkron motorların kullanımı yaygın olmasıyla birlikte gelişen teknolojiye paralel olarak çok fazlı (3'ten fazla) asenkron motor kavramı ortaya çıkmıştır. Çok fazlı asenkron motorlar geleneksel üç fazlı asenkron motorlara göre birçok avantaja sahiptir. Özellikle yüksek güç gerektiren uygulamalarda, elektrikli ve hibrit araçlarda, hava ve deniz taşıtlarında vs. kullanılmaktadır. Çok fazlı asenkron motorlar genellikle inverterlerle beslenmektedir. İnverterin çıkışındaki harmonikler o dalganın kalitesinin belirlenmesindeki en temel unsurdur. Harmonik bozulmaların önüne geçmek için çeşitli yöntemler kullanılarak çıkış dalga şeklinin sinüse yakın olması amaçlanmaktadır. Bu yöntemlerden biri de sinüzoidal darbe genişlik modülasyon tekniğidir. Bu çalışmada sinüzoidal darbe genişlik modülasyon yöntemi ile beş fazlı asenkron motorun kontrolü yapılmıştır. Beş fazlı asenkron motora ait matematiksel denklemler kullanılarak, beş fazlı asenkron motorun simulink model uygulaması d-q referans çerçevesinde oluşturulmuştur. Benzetim modelleri MATLAB/Simulink paket programında gerçekleştirilmiştir.
Silicone is often used in environments where water repellency is an advantage. Contact with water promotes the adhesion of microorganisms and biofilm formation. Depending on the application, this may increase the possibility of food poisoning and infections, the material's degrading appearance, and the likelihood of manufacturing defects. The prevention of microbial adhesion and biofilm formation is also essential for silicone-based elastomeric foams, which are used in direct contact with human bodies but are often difficult to clean. In this study, the microbial attachment in and the retention from the pores of silicone foams of different compositions is described and compared to those of commonly used polyurethane foams. The growth of the gram-negative Escherichia coli in the pores and their leaching during wash cycles is characterised by bacterial growth/inhibition, adhesion assay, and SEM imaging. The structural and surface properties of the materials are compared. Despite using common antibacterial additives, we have found that non-soluble particles stay isolated in the silicone elastomer layer, thus affecting surface microroughness. Water-soluble tannic acid dissolves into the medium and seems to aid in inhibiting planktonic bacterial growth, with a clear indication of the availability of tannic acid on the surfaces of SIFs.
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